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RBM4_2xRRM-ZNF_pGEX

Plasmid

#135154

Purpose

Recombinant expression of RBM4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM4 (RBM4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHG6

Plasmid

#188254

Purpose

Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

Depositor

Hong Gao, Maggie Smith

Article

Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

act1p/actII-orf4

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PthrC3_8

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMVV098

Plasmid

#215304

Purpose

Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PPhIF

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCC25

Plasmid

#177319

Purpose

Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

Depositor

Rafal Tomecki

Article

Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

Insert

TtCutA 240-603

Use

Tags

6xHis-SUMOTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GST PTBP1 isoform 4

Plasmid

#108594

Purpose

Recombinant GST tagged protein production

Depositor

Chris Smith

Article

Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

Insert

PTBP1 isoform 4 (PTBP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

isoform 4 (isoform a)

Promoter

tac

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S18

Plasmid

#127827

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Use

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-His10Smt3-gamma-toxin

Plasmid

#197000

Purpose

Bacterial expression plasmid for gamma toxin tRNA endonuclease

Depositor

Dragony Fu

Article

Lentini et al RNA. 2018 May;24(5):749-758. doi: 10.126

Insert

Gamma toxin

Use

Tags

His10-Smt3

Expression

Bacterial

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-SBP-TEV

Plasmid

#36943

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Tags

6xHistag-SBP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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BRD9

Plasmid

#39012

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD9 (BRD9 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Article

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Use

Tags

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRU1099

Plasmid

#14464

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

unstable gfp-mut3.1 AAV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pEB1-coSCFP3A

Plasmid

#104015

Purpose

Plasmid encoding coSCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coSCFP3A

Use

Low copy

Tags

Expression

Bacterial

Mutation

Same protein sequence as SCFP3A (codon optimized …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA3(GAT)

Plasmid

#79436

Purpose

Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA3 (GGA3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 107-286

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pKL10-mRuby-iATPSnFR1.0 bb118

Plasmid

#158980

Purpose

Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mruby-iATPSNfR1.0

Use

Tags

mruby3

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#102557

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-165

Plasmid

#118577

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAV10.KH

Plasmid

#63208

Purpose

pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ205

Plasmid

#104586

Purpose

Express SNAP-6His-CBD

Depositor

New England Biolabs, Yinhua Zhang

Insert

SNAP-6His-CBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pTNSQCas

Plasmid

#219434

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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