-
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pCDFDuet-trAtCCD7-OsD27(pYL726 )
Plasmid#178285PurposeCo-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from ArabidopsisDepositorInsertsOsD27
trAtCCD7
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-trAtCCD8(pYL735)
Plasmid#178286PurposeExpresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
HBT-GCaMP6-HA
Plasmid#107433PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertGCaMP6S
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
FEV_pet28a
Plasmid#131668PurposeBacterial expression of ETS gene FEVDepositorInsertFEV (FEV Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-DmMe31B_G
Plasmid#146381PurposeBacterial Expression of DmMe31BDepositorInsertDmMe31B (me31B Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHSTC97
Plasmid#124352PurposePlasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxinDepositorInsertNpuDnaBΔ283 mini-intein
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationDeletion of 283-residue endonuclease domainPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB070.iodo.5
Plasmid#99397PurposeExpression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanineDepositorInsertPN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
UseTagsExpressionBacterialMutationPromoterPN25, Ptet, and PproKAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-SdB-T
Plasmid#87307PurposeExpresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.DepositorInsertStabilized dimeric Broccoli aptamer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
UseTags3xFLAG-tag and His14-tagExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p109.1
Plasmid#44518DepositorInsertHistone 4
UseTagsGSTExpressionBacterialMutationcontains full length (aa 1-102)PromoterAvailable sinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-Kaede (Kaede-A69T)
Plasmid#99228PurposeBacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).DepositorInsertpr-Kaede
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
IL13 promoter luciferase
Plasmid#11784DepositorInsertIL-13 promoter (IL13 Human)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUCBB-oriT
Plasmid#32556DepositorInsertoriT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDMX (23-111)
Plasmid#62064PurposeExpression of human MDM4 (residues 23-111) in e. coliDepositorInsertMDM4 (MDM4 Human)
UseTagsGSTExpressionBacterialMutationcontains residues 23-111PromotertacAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP1
Plasmid#71465PurposeEncodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 1 (SENP1 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET mCitrine LIC cloning vector (u-mCitrine)
Plasmid#29771DepositorTypeEmpty backboneUseTagsmCitrineExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3187 HPV-16 E2
Plasmid#10846DepositorInsertHPV-16 E2 (E2 Human papillomavirus)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only