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Plasmid#20743DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSW002
Plasmid#111248PurposeBroad host-range bacterial vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL4529 (pTnpA)
Plasmid#210869PurposeExpresses GstTnpA from pLac promoterDepositorInsertGstTnpA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
MYH11-gRNA1
Plasmid#132587PurposegRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )DepositorInsertMYH11-gRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Tn5
Plasmid#127917PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.DepositorInsertTn5
UseTagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7Available sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACM4
Plasmid#49797PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBHSTC104
Plasmid#124356PurposePlasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxinDepositorInsertgp41-1 N-intein and C-intein (bicistronic)
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJS124
Plasmid#207593PurposeGFP-BIB H20A expressionDepositorInsertGFP-BIB H20A
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDWJ27
Plasmid#207604PurposeGFP-BIB expressionDepositorInsertGFP-BIB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
UseTagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alaninePromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)
Plasmid#86933PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTagsBiotin, His6, MBP, Tev Recognition Sequence, and …ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-17X-PTDH
Plasmid#166786PurposePhosphite dehydrogenase for NAD(P)H regenerationDepositorInsertPhosphite dehydroenase
UseTagsHis6ExpressionBacterialMutationA196R, T201S, A328T, E352N, and C356D- please see…PromoterT7Available sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MEK1-DD
Plasmid#47576PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1DepositorInsertMEK1-DD (Map2k1 Mouse)
UseTags3xHA and GSTExpressionBacterialMutationSer218 to Asp, Ser221 to AspPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorInsertmEGFP-H1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only