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Plasmid#171235PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)DepositorInsert2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
R6K_tac_mCherry_ChInt
Plasmid#187382Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherryDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD-LBD
Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-AR-LBD
Plasmid#171239PurposeMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)DepositorInsert1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD-DBD
Plasmid#171236PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJLG039
Plasmid#192982PurposeBHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoterDepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRham-CALM1
Plasmid#182500PurposeBacterial expression of the Homo sapiens calmodulin, CALM1DepositorInsertCALM1 (CALM1 Human)
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBBR1k-J23107-GFPuv
Plasmid#106389PurposepBBR1k-GFPuv with Anderson promoter J23107 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJLG038
Plasmid#192981PurposeBHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoterDepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvLEAM
Plasmid#90033PurposeTo express His-tagged RvLEAM in bacteriaDepositorInsertRvLEAM
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pREDCas9
Plasmid#71541PurposeConstitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
lambda red genes
plasmid curing system
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CTB
Plasmid#141166PurposeExpresses recombinant Cholera Toxin B SubunitDepositorInsertCTB
UseTagsExpressionBacterialMutationNo secretion signalPromoterT7Available sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD239-1g-2
Plasmid#90959PurposeExpresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoterDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23109, and PttrB185-269Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD238-1a
Plasmid#90958PurposeExpresses S. baltica TtrS constitutivelyDepositorInsertTetrathionate sensor kinase
UseTagsExpressionBacterialMutationPromoterBba_J23109Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF-FGE
Plasmid#102615PurposeInducible expression of Formylglycine-generating enzymeDepositorInsertFormylglycine-generating enzyme
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only