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pUM

Plasmid

#64176

Purpose

harbours marker genes URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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WT C-His Ana GvpC in pET28a

Plasmid

#85732

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET-X(4130)B1

Plasmid

#168018

Purpose

Expresses X(4130)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(4130)B1-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD4DARPin

Plasmid

#198294

Purpose

Produces PVCs retargeted with anti-CD4 DARPin D27.2

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBSMrnaSeph

Plasmid

#67507

Purpose

Express the tRNAMet scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
Hchk2 (kin-)-PUNI10

Plasmid

#41908

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Expression

Bacterial

Mutation

kinase dead (D347A)

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pJExpress414_GmPOPB-D661A

Plasmid

#92236

Purpose

Express D661A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-D661A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Aspartate 661 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pmMaple3-CAM

Plasmid

#101148

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple3

Expression

Bacterial

Promoter

pBAD

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCoofy44

Plasmid

#55185

Purpose

Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

Expression

Bacterial

Promoter

pPH (Polyhedrin promoter)

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pEND-164_Cacnes_pFAST

Plasmid

#225616

Purpose

Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

pFAST

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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MiCy-pBAD

Plasmid

#54565

Purpose

Localization: Protein Expression Vector , Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and MiCy

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pAIP4_3KT9

Plasmid

#234192

Purpose

Heterologous protein expression of aprataxin in Escherichia coli

Depositor

Christopher Bahl

Insert

3KT9 (APTX Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pYTK004

Plasmid

#65111

Purpose

Encodes ConL2 as a Type 1 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConL2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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nuc-mCherry-pTRIP

Plasmid

#163520

Purpose

Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

Depositor

Thomas Weber

Insert

nucleus targeted mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTmpKm

Plasmid

#176227

Purpose

A template plasmid for amplification of the cI-hok-neo cassette

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, neo

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Article

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Tags

His6 followed by a thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pRSETB

Plasmid

#48203

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pOPINS-UBE3C

Plasmid

#66711

Purpose

Expresses UBE3C HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KiAA0010 (UBE3C )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only