Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,690 results

Showing: 5141 - 5160 of 31690 results

  1. pNIC28-SENP1

    Plasmid
    #196354
    Purpose
    protein expression of the His-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    SENP1 catalytic domain
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    contains only E419-L644
    Promoter
    T7
    Available Since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    196354-plasmid-map-sequence-id-386265

    Enlarge

  2. 4QQG

    Plasmid
    #61948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP:JMC001-B06:C41570 (KAT5 Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid
    #41733
    Purpose
    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Bacterial
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    T7
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41733-plasmid-map-sequence-id-56305

    Enlarge

  4. pET28-6His-SUMO-Nsp10

    Plasmid
    #154959
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154959-plasmid-map-sequence-id-307848

    Enlarge

  7. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

    Enlarge

  20. CYP51A1 (3I3K)

    Plasmid
    #25161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP51A1_cw_09 (CYP51A1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 54-502
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5141 - 5160 of 31690 results