30,904 results

1. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

   Plasmid

   #190794

   Purpose

   Template vector to amplify pieces for creating multiplex sgRNA-arrays.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA-scaffold only
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_J23117

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-21a(+)/MBP-His

   Plasmid

   #38006

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   MBP (Maltose binding protein)
   

   Tags

   6xHIS

   Expression

   Bacterial

   Available Since

   Oct. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pBbE5a-Opto-Cre-Vvd

   Plasmid

   #134404

   Purpose

   Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAGGS-MCS-NLS-3XFLAG-V5

   Plasmid

   #196383

   Purpose

   Empty backbone vector to insert cDNA for strong expression in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Type

   Empty backbone

   Tags

   3xFLAG and V5

   Expression

   Bacterial and Mammalian

   Promoter

   CAGGs

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. NADK

   Plasmid

   #28268

   Depositor

   Cheryl Arrowsmith

   Insert

   NADK:HPC0AC-F12:C216828 (NADK Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains residues 68-426.

   Available Since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pAG GST Empty

   Plasmid

   #236735

   Purpose

   Empty bacterial expression plasmid for GST fusion proteins

   Depositor

   Melina Fan, Addgene Research Program

   Type

   Empty backbone

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAG GST EGFP

   Plasmid

   #236736

   Purpose

   Bacterial expression plasmid containing GST-tagged EGFP

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   EGFP
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   April 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pKEK3395

   Plasmid

   #234079

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   YukonOFP
   

   Expression

   Bacterial

   Promoter

   pJ23100-BsaI

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-22b(+) pCopA RBS30 mscarlet

   Plasmid

   #226370

   Purpose

   Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   mScarlet
   

   Tags

   6x Histag

   Expression

   Bacterial

   Promoter

   copA promoter (pCopA)

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

    Plasmid

    #198450

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGDP1 uvrA

    Plasmid

    #112892

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    uvrA-like protein
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGDP1 stat

    Plasmid

    #112886

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    stat
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS23·Pm-GFP

    Plasmid

    #122593

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_Nsp15

    Plasmid

    #156481

    Purpose

    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp15 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. KAT2B

    Plasmid

    #39002

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KAT2B (KAT2B Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pOPINB-HOIL-1 full-length (1–510)

    Plasmid

    #193858

    Purpose

    Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

    Depositor

    Bernhard Lechtenberg

    Article

    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

    Insert

    HOIL-1 (RBCK1 Human)
    

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    Codon optimised for expression in E. coli

    Promoter

    T7

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetOminiCMV-SKM

    Plasmid

    #136551

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIP4_1UXZ

    Plasmid

    #234166

    Purpose

    Heterologous protein expression of cellulase B in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1UXZ
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAC575

    Plasmid

    #127976

    Purpose

    AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    ble (bleomycin resistance marker)
    

    Use

    Fungal expression

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans trpC promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. NRAS

    Plasmid

    #55654

    Purpose

    expression of mouse NRAS

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    NRAS (Nras Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only