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32,672 results

Showing: 5141 - 5160 of 32672 results

  3. pJL CusS NarX Chimera

    Plasmid
    #225652
    Purpose
    Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    CusS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225652-plasmid-map-sequence-id-455039

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  4. pJL NrsS NarX Chimera

    Plasmid
    #225653
    Purpose
    Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NrsS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225653-plasmid-map-sequence-id-454026

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  5. pJL RssA NarX Chimera

    Plasmid
    #225654
    Purpose
    Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    RssA-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225654-plasmid-map-sequence-id-454025

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  6. pJL VanS NarX Chimera

    Plasmid
    #225655
    Purpose
    Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    VanS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225655-plasmid-map-sequence-id-454023

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  7. pQadd1F

    Plasmid
    #135660
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Expression
    Bacterial
    Promoter
    pFer
    Available Since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135660-plasmid-map-sequence-id-305687

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  11. BAZ2AA-c002

    Plasmid
    #53623
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    BAZ2AA
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    P43
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

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  19. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

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  20. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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Showing: 5141 - 5160 of 32672 results