32,866 results
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Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKm
Plasmid#176227PurposeA template plasmid for amplification of the cI-hok-neo cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, neo
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRelA'
Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
ExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyExpressionBacterialMutationL401GPromoterPalkSAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTnsABC
Plasmid#160031PurposeExpresses V. cholerae TnsABCDepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonExpressionBacterialMutationnoAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2_SEPT6
Plasmid#174497Purposebacterial co-expression of human SEPT2 and of human SEPT6DepositorAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP3-Halo
Plasmid#175310PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1
Plasmid#173932PurposeBacterial expression of ALC1DepositorAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA
Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f-2NLS
Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
ExpressionBacterialAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9+sgRNA expression vector precursor
Plasmid#171798PurposePICASSO: Library expression vector precursor for paired dCas9+sgRNA expressionDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterpLtetOAvailable SinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1400
Plasmid#172519PurposeExpression of SARS-COV-2 Nsp12 in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-PLproCS-MBP
Plasmid#169195PurposeTo express a PLpro substrate (GST-PLproCS-MBP) in E. coliDepositorInsertGST-PLproCS-MBP
TagsGST-MBP fusion (PLpro-cleavable) and His6 (intern…ExpressionBacterialPromotertac promoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC201plus
Plasmid#164227PurposeIntegrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterRhamnose-inducibleAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPaGE Pyl TAG FliC T248TAG
Plasmid#160089PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.DepositorInsertsPyrrolysyl tRNA(cua) (Methanosarcina mazei)
Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
Flagellin
UseSynthetic BiologyExpressionBacterialMutationChanged Threonine 248 to TAG stop-codon.PromoterPseudomonas aeruginosa Leu-tRNA native promoter a…Available SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ7
Plasmid#167136PurposeA tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterKanamycin promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#154138PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
ExpressionBacterialPromoterP_gyrAAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsExpressionBacterialPromoterPgyrAAvailable SinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only