33,194 results

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iYFP-Parkin Plasmid

Plasmid

#197107

Purpose

Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

Depositor

Dori Woods

Article

Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

Insert

parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)

Use

Retroviral

Tags

YFP

Expression

Bacterial and Mammalian

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53SS(1-393)

Plasmid

#200311

Purpose

Bacterial expression of human superstable p53 as His6GB1tev fusion

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (TP53 Human)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

M133L, V203A, N239Y, N268D

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSC5

Plasmid

#188602

Purpose

Superior Conjugative Plasmid to Diverse Yeast

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET24b-BR1.4

Plasmid

#199436

Purpose

expresses BAmRed1.4 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMRed1.4

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET24b-Amg2

Plasmid

#199434

Purpose

expresses Amg2 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

Amg2

Tags

his tag

Expression

Bacterial

Mutation

L159K mutation

Promoter

T7

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-Aqp4-eGFP

Plasmid

#176747

Purpose

Expresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA

Plasmid

#176741

Purpose

Expresses membrane bound biotin ligase, BioID2, in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-13xLinker-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

Plasmid

#190790

Purpose

[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Inserts

Cas9
mazF-LVA

Use

CRISPR and Synthetic Biology

Tags

LVA

Expression

Bacterial

Mutation

silent mutations of BpiI-sites in original Cas9 g…

Promoter

PnrsB and Ptrc-[E*](104)

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYDE009

Plasmid

#194151

Purpose

Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

tetR-tetP-dcas9-rrnB T1-T7Te

Expression

Bacterial

Promoter

tetp

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Pramr-GFP

Plasmid

#194148

Purpose

Contains a RamR-regulated promoter expressing GFP

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GFP

Expression

Bacterial

Promoter

Pramr

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSUC01

Plasmid

#177204

Purpose

chromogenic reporter for gene knockout in Streptomyces

Depositor

Wenjun Guan

Article

Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

Insert

SaindC

Use

Synthetic Biology

Tags

NONE

Expression

Bacterial

Mutation

NONE

Promoter

TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-BETAipi-ispA/dxs

Plasmid

#178302

Purpose

Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

ispA
dxs

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-pHScarlet

Plasmid

#191258

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet

Depositor

Robert Campbell

Insert

pHScarlet

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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T7-LacO-GFP_ColE1

Plasmid

#171648

Purpose

Expresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-LacO-GFPssrA-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD(V203D)

Plasmid

#190090

Purpose

Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD(V203D)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-165

Plasmid

#118577

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBA562

Plasmid

#186237

Purpose

pTet-RfxCas13d crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-AldA WT

Plasmid

#187976

Purpose

Expresses human aldolase A

Depositor

Audrey Lamb

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

AldA WT (ALDOA Human)

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pREDusk-AmpR-MCS

Plasmid

#188974

Purpose

For red light-repressed bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAB50

Plasmid

#101678

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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