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33,544 results

Showing: 5141 - 5160 of 33544 results

  1. pMP11

    Plasmid
    #215559
    Purpose
    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin
    Depositor
    Brian Pfleger
    Publication
    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10
    Inserts
    Cas9
    gRNA targeting pBR322 origin of replication
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23105 and tet
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    215559-plasmid-map-sequence-id-434132

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  4. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy3 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

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  5. SPLICSFAST-LY-MT-P2A

    Plasmid
    #214897
    Purpose
    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LY-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214897-plasmid-map-sequence-id-423483

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  6. SPLICSFAST-LDh-MT-P2A

    Plasmid
    #214896
    Purpose
    Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    214896-plasmid-map-sequence-id-423482

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  9. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Publication
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  10. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Publication
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  12. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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Showing: 5141 - 5160 of 33544 results