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pJJDuet30

Plasmid

#11962

Depositor

Hideo Iwai

Article

Zuger et al Nat Biotechnol. 2005 Jun . 23(6):736-40.

Insert

Ssp DnaE-N

Tags

GB1

Expression

Bacterial

Available Since

Sept. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC

Plasmid

#99641

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pttq18

Plasmid

#69122

Purpose

regulated expression of genes in Escherichia coli

Depositor

Michael Stark

Article

Stark et al Gene. 1987;51(2-3):255-67.

Type

Empty backbone

Expression

Bacterial

Promoter

tac

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pNIC28-BIRC3

Plasmid

#196366

Purpose

protein expression of the His-tagged full length protein

Depositor

Stefan Knapp

Insert

BIRC3/cIAP2

Tags

His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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SL-SunTag

Plasmid

#196919

Purpose

The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)::MBP-NL3F10H

Plasmid

#141291

Purpose

MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

MBP-NLUC3F10H

Use

Luciferase

Tags

Flag (x3), His (x10), and MBP

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2Q2

Plasmid

#15801

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2Q2 (UBE2Q2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pBAD/HisB-PAmCherry1

Plasmid

#31931

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

His

Expression

Bacterial

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

araB

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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BB-NIC-II-HisN-TET12

Plasmid

#45496

Depositor

Roman Jerala

Article

Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

Insert

TET12

Use

Synthetic Biology

Tags

6xHistidine tag

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pNHA7

Plasmid

#223649

Purpose

birA protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BioID_G118R

Tags

6His

Expression

Bacterial

Mutation

G118R

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Article

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMD19T-psba1-TetR-PL22-dCas9-SpR

Plasmid

#73223

Purpose

Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PL22

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAB094a

Plasmid

#79241

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PBAD rpoZ-HA4; PC araC

Expression

Bacterial

Promoter

PBAD; PC

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL7010

Plasmid

#136942

Purpose

Constitutive sfGFP expression (high), bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196)-Strep

Plasmid

#196208

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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IL-4 promoter luciferase

Plasmid

#12195

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-4 promoter (Il4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMLBAD

Plasmid

#32055

Purpose

Vector optimized for inducible gene expression in Burkholderia

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Type

Empty backbone

Expression

Bacterial

Promoter

Pbad

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-PH-PLCD1

Plasmid

#183675

Purpose

PtdIns(4,5)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLCD1 (PLCD1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 11-140

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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