32,967 results
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Plasmid#182320PurposeIt's an empty replication plasmid backbone in BacteroidesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only
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EGFP-mCherry-Ki67
Plasmid#183750PurposeExpresses EGFP-mCherry-Ki67DepositorAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN4
Plasmid#180285PurposeKanR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - KanR - CEN6/ARS4
ExpressionBacterial and YeastAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-A
Plasmid#177784PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-EGFP
Plasmid#182641PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
TagsBAP, EGFP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Opt WWP1
Plasmid#179327PurposeE. coli optimized expression of GST-fusion of human WWP1DepositorInsertWWP1 (WWP1 Human)
TagsGSTExpressionBacterialMutationCodon optimized for expression in E. coliAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AP1
Plasmid#179678PurposeAccessory plasmidsDepositorInsert[SD8] gIII
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-NLS-M-MLV-RT
Plasmid#181804PurposepET28a-NLS-M-MLV RTDepositorInsertNLS-M-MLV-RT
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)
Plasmid#181792PurposeExpresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1-GFP
Plasmid#180420PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.DepositorInsertGFP
ExpressionBacterialAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_35bpLinker
Plasmid#157790PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_35bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF7 HLA-B*07:02
Plasmid#180446PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*07:02
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF236 HLA-B*40:01
Plasmid#180463PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*40:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-array 8
Plasmid#175580PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-HA-GFP11
Plasmid#177719PurposeER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFPDepositorAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AF1521-c003
Plasmid#173111PurposeExpression of recombinant fusion protein in E. coliDepositorInsertAF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
TagsNanolucExpressionBacterialMutationKH-M1-L192Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXint129
Plasmid#178208PurposeEncodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.DepositorExpressionBacterialPromoterlacIAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only