33,228 results
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Plasmid#203681PurposeExpresses Cyan Fluorescent Protein (CFP) in Listeria monocytogenesDepositorInsertCyan Fluorescent Protein (CFP)
ExpressionBacterialAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-02
Plasmid#187837PurposeBackbone for translational reporter system in Fusobacterium nucleatumDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Cas7-11_crRNA
Plasmid#197612PurposeFor protein purification of Cas7-11DepositorInsertSUMO_TwinStrep_Cas7-11 with crRNA
ExpressionBacterialAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
ExpressionBacterialPromoterglnS' and lambda(ts) and proKAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Mm2
Plasmid#197100PurposeTo express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivatiDepositorInsertPylRS variant, Pyl tRNA, kanamycin resistance gene
ExpressionBacterialMutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
ExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialPromoteraraC and lppAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-Cas12m
Plasmid#192274PurposeEncodes MmCas12m under a constitutive promoterDepositorInsertMmCas12
UseCRISPRExpressionBacterialPromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sth1Cas9n cytosine base editor
Plasmid#194209PurposeSth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recordingDepositorInsertSth1Cas9-D9A nickase
UseCRISPRExpressionBacterialMutationChanged Aspartate 9 to Alanine.Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET.HaloTag.v7.AviTag
Plasmid#195486PurposeBacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.DepositorInsertHaloTag.v7.AviTag
Tags6xHIS and AviTagExpressionBacterialPromoterT7Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
iYFP-Parkin Plasmid
Plasmid#197107PurposeExpresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.DepositorInsertparkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
UseRetroviralTagsYFPExpressionBacterial and MammalianAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53SS(1-393)
Plasmid#200311PurposeBacterial expression of human superstable p53 as His6GB1tev fusionDepositorInsertp53 (TP53 Human)
TagsHis6-GB1-tevExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#188602PurposeSuperior Conjugative Plasmid to Diverse YeastDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24b-BR1.4
Plasmid#199436Purposeexpresses BAmRed1.4 in bacterial cellsDepositorInsertBAMRed1.4
Tagshis tagExpressionBacterialPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24b-Amg2
Plasmid#199434Purposeexpresses Amg2 in bacterial cellsDepositorInsertAmg2
Tagshis tagExpressionBacterialMutationL159K mutationPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Aqp4-eGFP
Plasmid#176747PurposeExpresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytesDepositorInsertAqp4-eGFP
UseAAVTagsGFPExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pramr-GFP
Plasmid#194148PurposeContains a RamR-regulated promoter expressing GFPDepositorInsertGFP
ExpressionBacterialPromoterPramrAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only