31,658 results
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Plasmid#194800PurposecCadC-GCN4 under the control of PtetDepositorInsertcCadC-GCN4
UseLuciferase and Synthetic BiologyExpressionBacterialAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBL069B
Plasmid#194802PurposecCadC-GCN4* under the control of PtetDepositorInsertcCadC-GCN4*
ExpressionBacterialAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-SUMO-MproC145A
Plasmid#172653PurposeBacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMODepositorInsertSARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Tags6xHis Tag and SUMOExpressionBacterialMutationCatalytic Cysteine 145 mutated to AlaninePromoterT7Available SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pet16b-Avi-ubiquitin_rbs_BirA
Plasmid#134897PurposeExpresses HIS-Avi-tagged ubiquitin for recombinant protein productionDepositorInsertsTags10HIS-AviTag-PreScission(3C)proteaseExpressionBacterialPromoterRBS and T7Available SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-YAP
Plasmid#166443PurposeBacterial expression of 6x His tagged YAPDepositorInsertYAP (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtLeuRS
Plasmid#31079DepositorInsertMature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
TagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …Available SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PFN1_Profilin
Plasmid#110028PurposeProtein expression and purification of PFN1_ProfilinDepositorInsertPFN1_Profilin (PFN1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM1
Plasmid#32881DepositorInsertMATR3 Matrin3 (MATR3 Human)
TagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 399-468PromoterCMVAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP30_Encap
Plasmid#86406PurposeExpresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 30aa CLP tag
Encapsulin
Tags6x HistidineExpressionBacterialPromoterT7Available SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
Tags6xHisExpressionBacterialPromoterPtacAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-7D12Gln13TAG
Plasmid#125264PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertGln13TAG 7D12
TagsHis-TagExpressionBacterialAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialPromoterPtrc coreAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNK
Plasmid#135193PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 aSyn C141
Plasmid#108866PurposeExpresses human WT alpha synuclein with a C-terminal cysteineDepositorInsertalpha synuclein (SNCA Human)
ExpressionBacterialMutationC141 mutation to allow C-terminal maleimide dye l…PromoterT7Available SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBb(B5)1k-GFPuv
Plasmid#106393PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL-hCul1-NTD
Plasmid#29518DepositorInsertCUL1 (CUL1 Human)
TagsThrombin Cleavage SiteExpressionBacterialMutationN-terminal 415 aaAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN1A-c001
Plasmid#175491PurposeProtein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET.HaloTag.v7.CoOp.Cys-less
Plasmid#195487PurposeBacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.DepositorInsertHaloTag.v7
Tags6xHISExpressionBacterialMutationC61T, C262VPromoterT7Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only