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Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorInsertGα11-LgB97 (GNA11 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorInsertEB1-GFP-6xHis (Mapre1 Mouse)
UseTagseGFP-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_BAP_his
Plasmid#210402PurposeFor expression of VHH with C-terminal biotin acceptor peptide and his tagDepositorTypeEmpty backboneUseTagsbiotin acceptor peptide-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his
Plasmid#210405PurposeFor expression of VHH with C-terminal his tagDepositorTypeEmpty backboneUseTagshis6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBJ5
Plasmid#167134PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-R-gfp
Plasmid#220927PurposeExpresses gfp using J23119 promoter and pgRNA_AT backboneDepositorInsertsuperfolder GFP
UseTagsFLAGExpressionBacterialMutationPromoterJ23119Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern/pRSETB
Plasmid#51969Purposebacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imagingDepositorInsertNano-lantern
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-L1T1L-NLuc
Plasmid#128942PurposeExpresses L1-NLuc geneDepositorInsertL1 minor core protein
UseTagsNanolucExpressionBacterialMutationPromoterT7 promotorAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEZY19
Plasmid#18668PurposepET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.DepositorTypeEmpty backboneUseGateway destination vectorTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKSJ376
Plasmid#103065PurposeExpresses colicin E1 with R domain deletion and ImmE1DepositorUseTagsExpressionBacterialMutationR (receptor-binding domain) deletion in colicin E…PromoterT7 lac (from pET) and T7 lac (from pET52-b)Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
KARS_pNIC-Bio3
Plasmid#153051PurposeExpression construct for producing Avi tagged Lysyl-tRNA synthetaseDepositorInsertKARS (KARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET StrepII-StrepII TEV LIC cloning vector (2RRT)
Plasmid#55215DepositorTypeEmpty backboneUseTags2xStrep II and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNRPA_Full-length_pGEX
Plasmid#135134PurposeRecombinant expression of SNRPA with dual-affinity tagDepositorInsertSNRPA (SNRPA Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23106-B0034m-E0040m-B0015)
Plasmid#197671PurposeExpresses GFP under the control of J23106 and B0034mDepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Opt WWP1
Plasmid#179327PurposeE. coli optimized expression of GST-fusion of human WWP1DepositorInsertWWP1 (WWP1 Human)
UseTagsGSTExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-SBiT_Strep-Tag-II
Plasmid#198750PurposeExpresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - SmallBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJDC60
Plasmid#157780PurposetdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.DepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterL5Available sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD_mCherry
Plasmid#187387Purposearabinose-inducible expression of mCherryDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only