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Plasmid#218366PurposeEmpty backbone for Tn7-site insertionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pUC18T-mini-Tn7T-hph-Para
Plasmid#218363PurposeEmpty backbone for Tn7-site insertion with araC-ParaBAD expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptol
Plasmid#218359PurposeEmpty vector with XylS-Pm expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptet1
Plasmid#218367PurposeEmpty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptol
Plasmid#218369PurposeEmpty backbone for Tn7-site insertion with XylS-Pm expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_(27F3)_FLAG
Plasmid#199615PurposeEncodes DARPin-FLAG 27F3 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27F3
UseTags8xHis and FLAGExpressionBacterialMutationPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJKW0957
Plasmid#119026PurposeE.coli expression plasmid for PmKOMT1DepositorInsertPmKOMT1
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPro33
Plasmid#17810DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1
Plasmid#136647PurposeExpression of SHBP1-GST fusion protein in E. coliDepositorInsertSH3BP1 (SH3BP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNA500
Plasmid#149576Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterUser-definedAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.1-c002
Plasmid#173087PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
UseTagsmCeruleanExpressionBacterialMutationQ162-N369, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor T19N
Plasmid#12152DepositorInsertRhoA Biosensor T19N (RHOA Human)
UseTags6xHisExpressionBacterial, Insect, and Mamm…MutationRhoA T19N (dominant-negative mutation)*PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28-T7-NlaCas3-NLS-6xHis
Plasmid#180214PurposeExpressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.ColiDepositorInsertNlaCas3c
UseTags6xHis and NLSExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP-T
Plasmid#103998PurposePlasmid encoding TagRFP-TDepositorInsertTagRFP-T
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
UseTagsFLAG purification tag and eGFPExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36γ
Plasmid#214345PurposeProtein overexpression in bacteriaDepositorInsertIL36G (IL36G Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 WT
Plasmid#13321DepositorInsertPINK1 kinase domain (PINK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 112-496PromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only