30,260 results

1. pRSET iGluSnFR3 v857

   Plasmid

   #175186

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v857
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-LIC

   Plasmid

   #26094

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICSFAST-LY-MT-P2A

   Plasmid

   #214897

   Purpose

   Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-LY-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM4Z-Fluc

   Plasmid

   #183474

   Purpose

   This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHyperTEV60

   Plasmid

   #228496

   Purpose

   10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   TEV Protease
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. mApple-pBAD

   Plasmid

   #54536

   Purpose

   Localization: Protein Expression Vector , Excitation: 568, Emission: 592

   Depositor

   Michael Davidson, Roger Tsien, Nathan Shaner

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Use

   Tags

   6xHis and mApple

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP3545

   Plasmid

   #46888

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PnisA

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPROEX Aqua

    Plasmid

    #42889

    Depositor

    Fabienne Merola

    Article

    Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

    Insert

    Aquamarine
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRBC-sfGFP wild type

    Plasmid

    #174075

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnEA-vH_His-TEV-SEPT2_SEPT6

    Plasmid

    #174497

    Purpose

    bacterial co-expression of human SEPT2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbA6c_PsulA_sfGFP

    Plasmid

    #224587

    Purpose

    SOS response reporter

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsulA

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. HA-HA-BI-AR

    Plasmid

    #171234

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAD1

    Plasmid

    #47697

    Purpose

    General cloning vector for use in E. coli or D. radiodurans

    Depositor

    Mary Lidstrom

    Article

    Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    repU

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-DAXX-His

    Plasmid

    #175782

    Purpose

    DAXX protein for bacteria expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB2-mKate2

    Plasmid

    #104009

    Purpose

    Plasmid encoding mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mKate2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, V45A, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_GNAI2_G-alpha

    Plasmid

    #109791

    Purpose

    Protein expression and purification of GNAI2_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI2_G-alpha (GNAI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge