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Showing: 4561 - 4580 of 28150 results

  1. SUMO-KTag

    Plasmid
    #51723
    Purpose
    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SUMO-KTag
    Use
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. RAB25

    Plasmid
    #26163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB25:HPC038-B06:C25773 (RAB25 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

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  6. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  7. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171928-plasmid-map-sequence-id-338570

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  10. pU6T7G

    Plasmid
    #71463
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)
    Depositor
    Willie Mark
    Article
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    human U6/phage T7
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    71463-plasmid-map-sequence-id-128761

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  13. pECTO

    Plasmid
    #67683
    Purpose
    pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureus
    Depositor
    Philippe Bouloc
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    67683-plasmid-map-sequence-id-176849

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  14. pGBW-m4046852

    Plasmid
    #145584
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145584-plasmid-map-sequence-id-292508

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  16. KE-009

    Plasmid
    #133441
    Purpose
    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4H (EIF4H Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133441-plasmid-map-sequence-id-267720

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  17. pET28a Gx-mNG-NL

    Plasmid
    #169106
    Purpose
    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - mNeonGreen - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169106-plasmid-map-sequence-id-335361

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Showing: 4561 - 4580 of 28150 results