pHH0103_VAV1-1/2
(Plasmid
#91355)
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PurposeProtein expression and purification of human SH3 domain construct VAV1-1/2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91355 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHH0103
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameVAV1-1/2
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SpeciesH. sapiens (human)
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Insert Size (bp)248
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Entrez GeneVAV1 (a.k.a. VAV)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sfi1 (unknown if destroyed)
- 3′ cloning site Not1 (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHH0103_VAV1-1/2 was a gift from Sachdev Sidhu (Addgene plasmid # 91355 ; http://n2t.net/addgene:91355 ; RRID:Addgene_91355) -
For your References section:
Comprehensive Analysis of the Human SH3 Domain Family Reveals a Wide Variety of Non-canonical Specificities. Teyra J, Huang H, Jain S, Guan X, Dong A, Liu Y, Tempel W, Min J, Tong Y, Kim PM, Bader GD, Sidhu SS. Structure. 2017 Oct 3;25(10):1598-1610.e3. doi: 10.1016/j.str.2017.07.017. Epub 2017 Sep 7. 10.1016/j.str.2017.07.017 PubMed 28890361