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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Tags

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET-His-Dre

Plasmid

#123130

Purpose

Bacterial expression plasmid for wild-type Dre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Dre recombinase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHG7

Plasmid

#188255

Purpose

Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection

Depositor

Maggie Smith, Hong Gao

Article

Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

act1p/actII-orf4

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-3-SARS-CoV-2-3CL

Plasmid

#168457

Purpose

Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

Depositor

David Ho, Sho Iketani, Alejandro Chavez

Article

Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

Insert

SARS-CoV-2 3CL protease (ORF1ab Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAbaMCi-GFP-gfp_pJMP2768

Plasmid

#208889

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-tdTomato-hphMX6

Plasmid

#105157

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

Insert

tdTomato-hphMX6

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAT-WT-HA-GFP11

Plasmid

#177719

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p405TEF1

Plasmid

#15968

Depositor

Nicolas Buchler, Fred Cross

Insert

TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

TEF1 promoter is between SacI and XbaI restrictio…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pQTEV-PSMD10

Plasmid

#31332

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pET-Cre-6xHis

Plasmid

#62939

Purpose

Expression of Cre-6xHis in bacterial of cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cre recombinase

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pXD69m1-TetW

Plasmid

#191246

Purpose

E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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L1-E309K

Plasmid

#15487

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-E309K (L1CAM Human)

Use

Tags

Expression

Bacterial

Mutation

Glutamic acid 309 changed to Lysine

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pBAD/His-miRFP703

Plasmid

#79985

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Use

Tags

Expression

Bacterial

Mutation

Promoter

araB

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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ykgMO-IGS-sfGFP

Plasmid

#207231

Purpose

Calprotectin sensing promoter with sfGFP reporter

Depositor

Arthur Prindle

Article

Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

ykgMO-IGS

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His-VCre

Plasmid

#123131

Purpose

Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

VCre recombinase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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V621 pGEX-4T-LP - Acceptor

Plasmid

#11715

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pAB085d

Plasmid

#79209

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ-opt (OR1); Ptet

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NylB_pET29b

Plasmid

#215414

Purpose

Expression construct for NylB gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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