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Plasmid#29590DepositorInsertHuntingtin-interacting protein 1 (HIP1 Human)
UseTagsHis and MycExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSSK WT p85 alpha flag
Plasmid#13429DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-myc
Plasmid#218191PurposeBacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
UseTagsNSP4 signal secretion peptide and mycExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB076i5
Plasmid#79199PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2+3); Ppro1Available sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
AlphaB
Plasmid#124524PurposepColE1_AlphaBDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
AlphaD
Plasmid#124526PurposepColE1_AlphaDDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172503PurposeEncodes the DisCas7-11 protein only for bacterial recombinant protein expression for purificationDepositorInsertDisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3
Plasmid#110099PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage siteDepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_Nef_MCS2
Plasmid#66079PurposeSingle vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertNef
UseTags6xHis and HRV 3C SiteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
UseTags(-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD_GFP11_T7LysH17A
Plasmid#59591PurposeExpression vector for solubility screening of proteins or protein fragments using a split-GFP assay.DepositorTypeEmpty backboneUseTags6xHis-tag and GFP11ExpressionBacterialMutationPromoterpARAAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
UseTagsExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQFmcs-2H12.D11
Plasmid#221183PurposecdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)DepositorInsertcdGreen2
UseTagsExpressionBacterialMutationPromoterPQ5 (cumate-inducible)Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pQFmcs-2H12.D11-scarREF
Plasmid#221184PurposecdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)DepositorInsertscdGreen2
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterNone (same as cdGreen2) and PQ5 (cumate-inducible)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv1KA_amilCP
Plasmid#204054PurposeType TU2-a backbone with kanamycin resistance, pSC101-var1 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv2KA_amilCP
Plasmid#204053PurposeType TU2-a backbone with kanamycin resistance, pSC101-var2 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV298
Plasmid#204957PurposeAMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection markerDepositorInsertsMad7
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_SKA_amilCP
Plasmid#204055PurposeType TU2-a backbone with kanamycin resistance, pSC101 (WT) ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only