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Plasmid#119268Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD434-SR mouse Alpha-synuclein
Plasmid#89073Purposebacterial expression of mouse Alpha-synucleinDepositorInsertAlpha-synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBBR1-kan-Ptet-plu-gamma-ET_rhi145
Plasmid#180427PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ET_rhi145
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-ELP[AV-60]
Plasmid#67012PurposeExpresses malE-ELP[AV-60] in E. coliDepositorInsertmalE-ELP[AV-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGL821-8
Plasmid#58224PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
UseTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSRKBB-empty
Plasmid#59449PurposeContains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotermodified lacAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
bZiPro
Plasmid#86846Purposeexpress NS2B-NS3 protease in E.coliDepositorInsertsNS3 1-177
NS2B45-96
UseTagsa hexahistidine tag and a thrombin cleavage siteExpressionBacterialMutationNS2B cofactor region (residues 45–96) and NS3 pro…PromoterT7Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCis GFPuv-SS Himar1 A7
Plasmid#84686PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-thr-RAF1-RBD
Plasmid#86033PurposeExpresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activationDepositorInsertRAF1 RBD (Ras-binding domain) (RAF1 Human)
UseTagsGSTExpressionBacterialMutationContains residues 1-149PromoterT7Available sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-3WJdB-T
Plasmid#87308PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.DepositorInsertThree-way junction dimeric Broccoli aptamer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEA183
Plasmid#187873PurposeExpresses eGFP under the virB promoter.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterAgrobacterium virB promoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
6MIW
Plasmid#127291PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertHUWE1 (HUWE1 Human)
UseTags6xHis-TEV (MHHHHHHSSGRENLYFQG)ExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
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6xHis-MBP-BrCas12b
Plasmid#182276PurposeExpresses recombinant BrCas12bDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis, T7, Thrombin siteExpressionBacterialMutationPromoterT7Available sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTn7(Gm)PA1/04/03-ecfp-a
Plasmid#111619PurposeeCFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-ecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-3xHA-SAPAP3
Plasmid#190200PurposeExpresses SAPAP3 protein fused with an HA tag at the N-terminus in astrocytesDepositorInsert3xHA-SAPAP3 (Dlgap3 Mouse)
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMTEZ-TeTxLC
Plasmid#32640DepositorInsertTetanus toxin light chain
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only