bZiPro
(Plasmid #86846)

• Purpose: express NS2B-NS3 protease in E.coli
• Depositing Lab: Dahai Luo
• Publication: Zhang et al Science. 2016 Dec 8. pii: aai9309.

Backbone

• Vector backbone: pETDUET-1
• Backbone manufacturer: Novagene
• Backbone size w/o insert (bp): 5420
• Total vector size (bp): 6107
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: NS3 1-177
• Species: Synthetic; Zika virus
• Insert Size (bp): 534
• Mutation: NS3 protein (residues 1–177)
• GenBank ID: AEN75265.1
• Promoter: T7
• Tag / Fusion Protein:
  • a hexahistidine tag and a thrombin cleavage site (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: pET Upstream Primer
• 3′ sequencing primer: DuetDOWN1 Primer

Gene/Insert 2

• Gene/Insert name: NS2B45-96
• Species: Synthetic; Zika virus
• Insert Size (bp): 159
• Mutation: NS2B cofactor region (residues 45–96)
• GenBank ID: AEN75265.1
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: DuetUP2 Primer
• 3′ sequencing primer: T7R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was jointly developed at the Lee Kong Chian School of Medicine, Nanyang Technological University, and at the Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry