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Showing: 4041 - 4060 of 28414 results

  1. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  3. pGEX6P1 METTL3

    Plasmid
    #200731
    Purpose
    Bacterial expression of GST-Tagged codon optimized human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Use
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200731-plasmid-map-sequence-id-401808

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  9. ACAT1

    Plasmid
    #25510
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ACAT1 (ACAT1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. PTPN5

    Plasmid
    #39169
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Use
    Tags
    GST-PreScission
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pCDF-5xT7-TtCsm

    Plasmid
    #128572
    Purpose
    For expression of T. thermophilus Csm in E. coli (Csm1-5)
    Depositor
    Jennifer Doudna
    Article
    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:
    Inserts
    Csm1
    Csm2, Csm3, Csm4, Csm5
    Use
    Tags
    HRV 3C protease cleavage site and 10xHis tag on C…
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli and Cod…
    Promoter
    T7 promoter, lac operator
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128572-plasmid-map-sequence-id-252577

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  16. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4041 - 4060 of 28414 results