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Plasmid#153520PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.DepositorInserttFucci(SA)5.
UseTagsAzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt-scarRef
Plasmid#221150PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)DepositorInsertscdGreen2.1
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2.1)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTNS2
Plasmid#64968PurposeTn7 transposase expressionDepositorInserttnsABCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdTomato-pBAD
Plasmid#54856PurposeLocalization: Protein Expression Vector , Excitation: 554, Emission: 581DepositorTypeEmpty backboneUseTags6xHis and tdTomatoExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-pAG-Hia5
Plasmid#174373PurposePlasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein AG - hia5
UseTags6xHis and Protein A/GExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEVS104
Plasmid#207393PurposeConjugal helper plasmidDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ8-Ptac
Plasmid#74064PurposeIPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV LIC cloning vector (1B)
Plasmid#29653DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOlyA(E69A)
Plasmid#132392PurposeMutant version of OlyA that senses sphingomyelin without or with cholesterolDepositorInsertOstreolysin A point mutant (E69A)
UseTagsHis6 tagExpressionBacterialMutationMutations: C62S, E69A, C94S, S151CPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin WT
Plasmid#12647DepositorInsertUbiquitin - wild type (UBC Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE2D3
Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorInsertHis-Tev-UbE2D3 (UBE2D3 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with TEV cleavageExpressionBacterialMutationPromoterT7-lacOAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16b-PCNA
Plasmid#134898PurposeBacterial expression and purification of human PCNADepositorInsertPCNA (PCNA Human)
UseTagsuntaggedExpressionBacterialMutationfully synthetic, codon optimized for bacterial ex…PromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_N
Plasmid#167255PurposeBacterial expression of Nucleocapsid protein (residues 1-419)DepositorInsertN protein (residues 1-419) (N SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL3287
Plasmid#139750PurposeBase Cas12a CRISPRi plasmidDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BPK764
Plasmid#65767PurposeBacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNADepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
UseCRISPRTags3x FLAG and NLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD48-daf-2 RNAi
Plasmid#34834DepositorInsertdaf-2 (daf-2 Nematode)
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ack
Plasmid#62654PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expressionDepositorInsertexo, beta, gam, sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only