30,260 results

1. elavl3:hArr-TEV;he1.1:YFP

   Plasmid

   #218855

   Purpose

   D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-RNF126

   Plasmid

   #138643

   Purpose

   Bacterial expression of GST-RNF126 fusion protein

   Depositor

   Jane McGlade

   Article

   Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

   Insert

   RNF126 (RNF126 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Use

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSecUAG-Evol2

   Plasmid

   #163148

   Purpose

   Expresses machinery necessary for selenocysteine incorporation at UAG codons

   Depositor

   Dieter Söll

   Article

   Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

   Inserts

   allo-tRNA UTu2D
   thioredoxin
   Selenophosphate synthase
   Selenocysteine synthase
   Cysteine desulfurase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 32 switched to Selenocysteine (UAG), Cys…

   Promoter

   EM7, araBAD, native As SelD promoter, and native …

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

   Plasmid

   #218860

   Purpose

   10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hGCG-ICAM(1235)-nrxn1b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-his-3C_Tau352

   Plasmid

   #100092

   Purpose

   Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

   Depositor

   Nikolai Sluchanko

   Article

   Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

   Insert

   Fetal microtubule-associated protein tau (MAPT Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   wild type sequence

   Promoter

   T7

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Xla.Tubb2:hArr-TEV;he1.1:eYFP

   Plasmid

   #218859

   Purpose

   Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC18-mini-Tn7T-Gm-lacZ

   Plasmid

   #63120

   Purpose

   mini-Tn7 lacZ transcriptional fusion vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pET-3c-rhVTN_FL

   Plasmid

   #30223

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin without deletions (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No deletions

   Promoter

   Available since

   May 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. YopH (residues 164-468)

    Plasmid

    #79749

    Purpose

    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    YopH (residues 164-468)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218857

    Purpose

    Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Use

    Tags

    QF fusion protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. elavl3:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218852

    Purpose

    D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Use

    Tags

    QF fusion protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDB179

    Plasmid

    #91960

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBb(RSF1010)1k-GFPuv

    Plasmid

    #106395

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TXNRD1

    Plasmid

    #38863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TXNRD1 (Txnrd1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDM069

    Plasmid

    #216821

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold2
    

    Use

    Tags

    RIPGLIN and VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD33-mf-lon

    Plasmid

    #21867

    Depositor

    Robert Sauer

    Article

    Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

    Insert

    Lon (Mfl404 Mesoplasma florum)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all TGA codons changed to TGG codons

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge