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Showing: 21 - 40 of 27885 results
  1. tFucci(SA)5

    Plasmid
    #153520
    Purpose
    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.
    Depositor
    Insert
    tFucci(SA)5.
    Use
    Tags
    AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Inserts
    cdGreen2.1
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Insert
    protein AG - hia5
    Use
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pZ8-Ptac

    Plasmid
    #74064
    Purpose
    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pET28-MHL

    Plasmid
    #26096
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Hexahistidine tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pMCSG53_N

    Plasmid
    #167255
    Purpose
    Bacterial expression of Nucleocapsid protein (residues 1-419)
    Depositor
    Insert
    N protein (residues 1-419) (N SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 27885 results