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Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …PromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pET-PfuX7
Plasmid#182364PurposeExpresses PfuX7 DNA polymeraseDepositorInsertPfuX7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationV93QPromoterT7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSU10-30-50-100
Plasmid#173838PurposeCoexpresses 10, 30, 50 and 100 kD Penn State ladder proteinsDepositorInsertSTRHSTPAB
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU20-40-60-80
Plasmid#173839PurposeCoexpresses 20, 40, 60 and 80 kD Penn State ladder proteinsDepositorInsertSTRHSTPABPACCBPv3
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-pBAD
Plasmid#54622PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and mEGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAL-7xHis-TEV-hexa
Plasmid#222868PurposeExpresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
UseTags7xHis and MBPExpressionBacterialMutationC19V, L56V, C110V, C130S, S135G, S219V. Insert in…PromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
plRL19
Plasmid#163634PurposeL5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).DepositorInsertL5 Integrase
UseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP) and Tet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
UseTagsExpressionBacterialMutationPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GluRS-His
Plasmid#124109PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutamyl-tRNA ligase (gltX Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mClover3
Plasmid#74236PurposeConstitutive expression of mClover3 in bacteriaDepositorHas ServiceCloning Grade DNAInsertmClover3
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPSU250
Plasmid#173837PurposeExpresses 250 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R*
Plasmid#189852PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R*-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbS5a-Opto-Cre-Vvd-2
Plasmid#160400PurposeUpdated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd-2
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
UseTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbAW4k-loxP-TT-loxP-mRFP1
Plasmid#134405PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.DepositorInsertW4-loxP-TT-loxP-mRFP1
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterW4 (modified T7A1)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-eyfp
Plasmid#65031PurposeGmR mini-Tn7 vector, mobilizable, for YFP tagging bacteriaDepositorInserteyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only