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PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET co-transformation cloning vector (13S-A)
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Backbone manufacturerScott Gradia (Addgene plasmid # 48323)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameYopH (residues 164-468)
- Promoter T7
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YopH (residues 164-468) was a gift from John Chodera & Nicholas Levinson & Markus Seeliger (Addgene plasmid # 79749 ; http://n2t.net/addgene:79749 ; RRID:Addgene_79749) -
For your References section:
An open library of human kinase domain constructs for automated bacterial expression. Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD. Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081. 10.1021/acs.biochem.7b01081 PubMed 30004690