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Plasmid#64840PurposeA lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.DepositorInsertNkx2.2 (Nkx2-2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-msfYFPBb
Plasmid#118233PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFPDepositorInsertflagellin promoter driven msfYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCF5 HLA-A*01:01
Plasmid#180448PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*01:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPT119
Plasmid#85526PurposeRepressilator without degradation tags, with integrated reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPEPZ-sgRNAclone
Plasmid#141090PurposeThis vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.DepositorInsertmCherry
UseCRISPRTagsExpressionBacterialMutationPromoterp3Available sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-RecETh1h2h3h4_agrob6
Plasmid#180428PurposeGenome engineering of AgrobacteriumDepositorInsertRecETh1h2h3h4_agrob6
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pET151-hUCH37 (ISF1)
Plasmid#61929PurposeExpression of hUCH37 (ISF1) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-gst-xr
Plasmid#214761PurposeXylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coliDepositorInsertglutathione S-transferases fused with xylose reductase
UseTagsGlutathione S-transferasesExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mLemon
Plasmid#191256PurposeBacterial expression plasmids for pH sensitive fluorescent protein mLemonDepositorInsertmLemon
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
UseTagsHis tag and LAA degradation tagExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pE6n
Plasmid#17460Purposemodified pENTR vector for N-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
LB322 (Bcl-2 from ATG to -3934)
Plasmid#15381DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -3934 with both P1 and…PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYPet-His
Plasmid#14031DepositorInsertYPet
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LolT_C41S
Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P66-ss
Plasmid#137045PurposeE. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66949.1 (p66 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P66: lacks the P66 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-Cdc42-wt
Plasmid#12969DepositorInsertCdc42 (CDC42 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-4
Plasmid#13001DepositorInsertTSP-4 (THBS4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only