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Showing: 3601 - 3620 of 28411 results

  4. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

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  5. pAM5085

    Plasmid
    #85122
    Purpose
    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    Use
    Tags
    ECFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85122-plasmid-map-sequence-id-180108

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  12. pGEX4T1-Opt WWP1

    Plasmid
    #179327
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1
    Depositor
    Arthur Haas
    Insert
    WWP1 (WWP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Available since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179327-plasmid-map-sequence-id-358706

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  15. pTXB1-sfGFP

    Plasmid
    #51562
    Purpose
    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    superfolder GFP
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51562-plasmid-map-sequence-id-78800

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  16. pCN-mBFP

    Plasmid
    #63847
    Purpose
    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.
    Depositor
    Howard Salis
    Article
    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10
    Insert
    mBFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter and RBS was swapped. His-tag was removed…
    Promoter
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63847-plasmid-map-sequence-id-106570

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  18. RHOA

    Plasmid
    #73231
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOA (RHOA Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00071:Ras 7-180
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73231-plasmid-map-sequence-id-136222

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  19. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Use
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

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Showing: 3601 - 3620 of 28411 results