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Plasmid#195055PurposeExpresses SUF operon from B. subtilis from IPTG-inducible promoterDepositorInsertsufCDSUB
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-eCFP
Plasmid#78476PurposeBroad range bacterial fluorescence labeling (ECFP)DepositorInserteCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
5WCI (MYST1)
Plasmid#101269PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMYST1 (KAT8 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
GST PTBP1 isoform 4
Plasmid#108594PurposeRecombinant GST tagged protein productionDepositorInsertPTBP1 isoform 4 (PTBP1 Human)
UseTagsGSTExpressionBacterialMutationisoform 4 (isoform a)PromotertacAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNM262
Plasmid#128412PurposeVanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseSynthetic BiologyTagsamyE SPExpressionBacterialMutationPromoterPspank(V)Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2E
Plasmid#75291PurposeExpresses proteins in the cytoplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P1-3xMBT
Plasmid#46987PurposeInducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
UseTagsGST and PreScission cleavage siteExpressionBacterialMutationcontains amino acids 190–530 of accession NP_0562…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p406GAL1
Plasmid#17437DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.7
Plasmid#78176PurposeExpresses rsGreen0.7 in E. coli strains.DepositorInsertrsGreen0.7
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE152
Plasmid#118502Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.1.t1
Plasmid#73545PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.1.t1
pAcFRS.1.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorInsertsP gene
M gene
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TadCBE-Cas9NG
Plasmid#225093PurposePlasmid for bacterial purification of codon-optimized TadCBE-Cas9NGDepositorInsertTadCBE-Cas9NG
UseCRISPRTags8xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP555
Plasmid#222722PurposesfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)DepositorTypeEmpty backboneUseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC130
Plasmid#216364PurposexhNup98-Nb3i Nanobody productionDepositorInsertxhNup98-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
3TERM_bba_b0015_rev_chlo
Plasmid#163931PurposeL0 part - terminatorDepositorInsertfrom GenBank: KT364744.3, prokaryotic double terminator BBa_B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
SeAvs3
Plasmid#188791PurposeAvs3 from Salmonella enterica NCTC13175DepositorInsertSeAvs3
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMal_Abl-PRM 3R
Plasmid#112086PurposeBacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.DepositorInsertAbl-PRM-3R (ABL1 Human)
UseTagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMO719
Plasmid#117479PurposeDesulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only