-
Plasmid#134897PurposeExpresses HIS-Avi-tagged ubiquitin for recombinant protein productionDepositorInsertsUseTags10HIS-AviTag-PreScission(3C)proteaseExpressionBacterialMutationPromoterRBS and T7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pDule-Tet3.0
Plasmid#174080PurposeTetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertTet3.0 tRNA synthetase and tRNA
UseTagsExpressionBacterialMutationL270G, Y271L, N311G, C313APromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pBTK552
Plasmid#110618PurposeBTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE312B
Plasmid#128969PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxylS-PmAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
UseTags10xHis-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOVSD
Plasmid#81033PurposeMammalian expression of the super-dark state of LOV2DepositorInsertLOVSD
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450A, L514K, G528A, L531E, N538EPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD
Plasmid#171235PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)DepositorInsert2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP25-1A(sfGFP)
Plasmid#126972PurposeLevel 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF420
Plasmid#123659PurposeProduces cofactor F420 in E. coliDepositorInsertsCofD
FbiB
FbiD
FbiC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_R0040Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K48C
Plasmid#86588PurposeBacterial expression of Ubiquitin K48C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; K48CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKB01_tagRFP
Plasmid#69103PurposeExpresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInserttagRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDBamHI-Leu
Plasmid#38256DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP E17TAG
Plasmid#68295PurposeE17TAG GFP reporterDepositorInsertE17TAG GFP
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRADZ1
Plasmid#47698PurposeGeneral cloning vector for use in E. coli or D. radiodurans with lacZDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterrepUAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH3(2)-3R
Plasmid#112091PurposeBacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-3R (NCK1 Human)
UseTagsGSTExpressionBacterialMutationPromoterLacAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-5xT7-TtCsm
Plasmid#128572PurposeFor expression of T. thermophilus Csm in E. coli (Csm1-5)DepositorInsertsCsm1
Csm2, Csm3, Csm4, Csm5
UseTagsHRV 3C protease cleavage site and 10xHis tag on C…ExpressionBacterialMutationCodon optimized for expression in E. coli and Cod…PromoterT7 promoter, lac operatorAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
EID1-pGex-2TK
Plasmid#22689DepositorInsertE1A-like inhibitor of differentiation 1 (EID1 Human)
UseTagsGSTExpressionBacterialMutationwt cDNAPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
poRibo-T2
Plasmid#69347PurposeRibo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutationDepositorInsertoRibo-T
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only