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pCOLA-T7-WspR:D70E

Plasmid

#79164

Purpose

Expresses constitutively active WspR D70E under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Use

Tags

Expression

Bacterial

Mutation

D70E

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pQE31-rsEGFP2

Plasmid

#102879

Purpose

Expresses rsEGFP2 in bacterial cells

Depositor

Stefan Jakobs

Article

Grotjohann et al elife. 2012;1:e00248. doi: 10.7554/eLife

Insert

rsEGFP2

Use

Tags

6xHisTag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-nanoHER2-SpyTag003

Plasmid

#216312

Purpose

Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

nanoHER2-SpyTag003

Use

Tags

His6 tag and SpyTag003

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-RT003-GPM

Plasmid

#163383

Purpose

To express sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

GFP-PDGFRtm-2A-mCherry (GPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-LAC

Plasmid

#64965

Purpose

mini-Tn7 vector with Ptac expression cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pD-MSP1D1

Plasmid

#161607

Purpose

Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

D-MSP1D1

Use

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans were replaced b…

Promoter

T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHN764

Plasmid

#178819

Purpose

Expression of EndoH in E. coli

Depositor

Kazuhiro Abe

Insert

synthetic gene encoding endo-β-N-acetylglucosaminidase H

Use

Tags

HIs8 and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSALECT-EcoBam

Plasmid

#59705

Purpose

Folding reporter with ampicillin resistance as a readout

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pET28b-hUba2-His

Plasmid

#53117

Purpose

expression of full length human Uba2 with Cterminal His-tag in E.coli

Depositor

Frauke Melchior

Article

Werner et al Methods Mol Biol. 2009;497:187-99. doi:

Insert

UBA2 (UBA2 Human)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pKEN GFP mut2

Plasmid

#20409

Depositor

Stanley Falkow, Raphael Valdivia

Article

Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

S65A V68L S72A

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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KLC2

Plasmid

#40735

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KLC2 (KLC2 Human)

Use

Tags

6xHis TEV

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pAA3.stelN

Plasmid

#112011

Purpose

Expresses TelN enzyme

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

TelN

Use

Tags

strep and his

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTD-C_sfYFPTwinStrep

Plasmid

#45943

Purpose

expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic sfYFP (codon usage adapted to P.putida KT2440)

Use

Tags

Twin-Strep-tag

Expression

Bacterial

Mutation

Promoter

lacIq/Ptrc

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pJBEI-6410

Plasmid

#47049

Purpose

BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene

Use

Synthetic Biology; Bglbrick

Tags

Expression

Bacterial

Mutation

Promoter

PlacUV5

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET28sumo-MDA5-Full-length

Plasmid

#210826

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

Insert

MDA5 (IFIH1) Full Length Protein (IFIH1 Human)

Use

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRBBm59

Plasmid

#48115

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 gamma GST

Plasmid

#13280

Depositor

Michael Yaffe

Article

Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

Insert

14-3-3 gamma (Ywhag Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Smad3

Plasmid

#12630

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pTHMT_hnRNPF_FL

Plasmid

#139114

Purpose

express FL hnRNPF with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lba_Cas13a_WT

Plasmid

#91867

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbaCas13a (LbaC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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