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Plasmid#35050DepositorInsertSpyTagMBP
UseTagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119945PurposeExpresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-pAG-Hia5
Plasmid#174373PurposePlasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein AG - hia5
UseTags6xHis and Protein A/GExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1029
Plasmid#22066DepositorTypeEmpty backboneUseTagsT25-FlagExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pComb3XSS
Plasmid#63890Purpose3rd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTags6x His, HA tag, and geneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
IL2 pMalLP
Plasmid#41808DepositorInsertIL2 (IL2 Human)
UseTags3C protease site, 6x His, and Maltose Binding Pro…ExpressionBacterialMutationPromoterpTacAvailable sinceJan. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_50
Plasmid#109248PurposeincW BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R377
Plasmid#138247PurposeExpresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorInserthsf-1 (hsf-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119946PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pUCP20T-dsRedExpress
Plasmid#78470PurposeBroad range bacterial fluorescence labelingDepositorInsertdsRedExpress
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.RY
Plasmid#201006PurposepLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApRDepositorInsertsmcherry
sYFP2
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.RB
Plasmid#201007PurposepLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApRDepositorInsertsmcherry
TagBFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.YB
Plasmid#201008PurposepLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApRDepositorInsertssYFP2
TagBFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80L Hu IL-15-His tag
Plasmid#183256PurposeBacterial expression of human IL-15 with N-terminal His tagDepositorInsertIL-15 (IL15 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH
Plasmid#83907Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only