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Plasmid#217382PurposepTrc99A-mNeonGreen-mcdB for bacterial expressionDepositorInsertmcdB
UseTagsNeonGreenExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS*-PBBa_J23106-geneIII
Plasmid#134352PurposePhagemidDepositorInsertGene III
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCM291rfp
Plasmid#59775PurposepCMrfp with TrfA mutation (291E to G)DepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
UseTagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF1
Plasmid#70087PurposeEncode YTHDF1 with N-terminal GST tag for E. coli expressionDepositorInsertYTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-KKHSaCas9-BlastR
Plasmid#107189PurposeConfers constitutive expression of KKH SaCas9.DepositorInsertKKH SaCas9 (NEWENTRY )
UseTagsExpressionBacterialMutationE782K/N968K/R1015HPromoterChicken ß-ActinAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry
Plasmid#182390PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
OmpA-mCherry
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-UbcH5b/2419
Plasmid#11475DepositorInsertUbcH5b (UBE2D2 Human)
UseTagsExpressionBacterialMutationn/aPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pRF-PsrA
Plasmid#49368DepositorInsertPsrA repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-MjDim1
Plasmid#189936PurposeExpression of Mjdim1, a methyltransferase used in m6A-SAC-seq.DepositorInsertrsmA (MJ_RS00455 Methanocaldococcus jannaschii)
UseTags6x His tag, FLAG tag, SUMO tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKate2
Plasmid#68441PurposeTOPO construct expressing mKate2 for generation of GMAP-compatible geneDepositorInsertmKate2
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7
Plasmid#64953Purposemini-Tn7 base vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
repressilator (pZS1-lTlrLLtCL)
Plasmid#26489DepositorInsertLacI, tetR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express
Plasmid#103995PurposePlasmid encoding DsRed-ExpressDepositorInsertDsRed-Express
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE131
Plasmid#118485Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG
Plasmid#124941PurposeExpresion of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy1
Plasmid#43974DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only