Showing: 3081 - 3100 of 27093 results
-
Plasmid#52292Purposebacterial expression of human GlomulinDepositorInsertGlomulin (GLMN Human)
UseTagsGST and TEVExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
Split 'n coexpress CUL3-RBX1
Plasmid#52293Purposebacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1DepositorUseTagsGST, IRES, and TEVExpressionBacterialMutationcontains aa 1-309, contains aa 310-768, and conta…PromoterIRES and tacAvailabilityAcademic Institutions and Nonprofits only -
His-MBP human SPOP (aa 28-337)
Plasmid#52294Purposebacterial expression of human SPOP (aa 28-337) fused to His-MBPDepositorInsertSPOP (SPOP Human)
UseTagsHis-MBP and TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
His Puc (aa 1-393)
Plasmid#52295PurposeBacterial expression of His tagged Drosophila Puc fragmentDepositorInsertPuc (puc Fly)
UseTagsHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF2
Plasmid#52301PurposeEncode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
psbA2-PHLS (b)
Plasmid#52307PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpsbA2AvailabilityAcademic Institutions and Nonprofits only -
psbA2(noATbox)-PHLS (c)
Plasmid#52308PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationCAAATACA box in the psbA2 promoter to ggcgcgccPromoterpsbA2AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-PHLS (d)
Plasmid#52309PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-T7-g10-PHLS (e)
Plasmid#52310PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrc-T7AvailabilityAcademic Institutions and Nonprofits only -
cpc-PHLS (g)
Plasmid#52311PurposeReplaces the cpc operon in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterSynechocystis endogenous cpc-operon promoterAvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pFGL889
Plasmid#52321PurposeIntroducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFGL815
Plasmid#52322PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFGL815N
Plasmid#52323PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorTagsExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailabilityAcademic Institutions and Nonprofits only -
pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
UseTagsExpressionBacterial and YeastMutationPromoterREV1AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA
Plasmid#52347PurposeIntegration cassette to create p1-Short-UL*(TGA)DepositorInsertleu*
UseP1 integration vectorTagsExpressionBacterialMutationchanged S2F, A3V, Q180*PromoterUCS from pGKL2 ORF5AvailabilityAcademic Institutions and Nonprofits only -
Human Mevalonate Kinase (MK)
Plasmid#52429Purposebacterial expression of human Mevalonate Kinase (MK)DepositorInsertHuman Mevalonate Kinase (MVK Human)
UseTagsExpressionBacterialMutationsee commentsPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Human Phosphomevalonate kinase (PMK)
Plasmid#52430Purposebacterial expression of human Phosphomevalonate kinase (PMK)DepositorInsertPhosphomevalonate kinase (PMVK Human)
UseTagsHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 3081 - 3100 of 27093 results