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Addgene

pCWori-A13AMO-aaCPRct
(Plasmid #20117)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20117 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCWori
  • Backbone manufacturer
    P. Ortiz de Montellano, UCSF
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Amorphadiene oxidase and p450 reductase
  • Alt name
    AMO, CPR
  • Species
    A. annua

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCWori-A13AMO-aaCPRct was a gift from Jay Keasling (Addgene plasmid # 20117 ; http://n2t.net/addgene:20117 ; RRID:Addgene_20117)
  • For your References section:

    Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD. Nat Chem Biol. 2007 May . 3(5):274-7. 10.1038/nchembio875 PubMed 17438551