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Plasmid#154229PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
S5_EcoRV_Ef1a_AscI
Plasmid#128469PurposeEf1a promoterDepositorInsertEf1a promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
spNW80
Plasmid#173487PurposeExpresses spNW80 in Bl21DepositorInsertspNW80
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
B7-5
Plasmid#111493Purposetranslational initiation factor 1DepositorInserttranslational initiation factor 1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR0306 CcaCas13b His6-TwinStrep-SUMO
Plasmid#182687PurposeExpresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purificationDepositorInsertCcaCas13b
UseTagsSUMOExpressionBacterialMutationPromoterT7Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC001 - huLshC2C2-MBP for bacterial expression
Plasmid#79150PurposeExpresses human codon-optimized LshC2c2 for purification in E. coliDepositorInsertC2c2
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-B-iRFP
Plasmid#31855Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorInsertiRFP
UseTagsExpressionBacterialMutationS13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…PromoteraraBADAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23111
Plasmid#113149PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmGFP
Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
UseTags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HeLa full-length MLCK-GFP
Plasmid#46316DepositorInsertHeLa long myosin light chain kinase (MYLK Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
B1-3
Plasmid#111465Purposetranslational release factor 3DepositorInserttranslational release factor 3
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
B1-5
Plasmid#111467Purposetranslational elongation factor TsDepositorInserttranslational elongation factor Ts
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B3-2
Plasmid#111472Purposetranslational elongation factor 4DepositorInserttranslational elongation factor 4
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2/HB
Plasmid#26557DepositorInsertpT2/HB
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
O-TREM2-2
Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL99
Plasmid#78577PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR systemDepositorTypeEmpty backboneUseStreptomyces expressionTagsExpressionBacterialMutationPromoterinducible PnitA-NitR systemAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
B7-1
Plasmid#111489Purposetranslational elongation factor GDepositorInserttranslational elongation factor G
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHN767
Plasmid#178884PurposeExpression of bacterial PNGaseDepositorInsertPeptide:N-glycosidase F
UseTagsGST, His8ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-fis
Plasmid#51557PurposeE. coli fis ORF cloned into pBAD24 expression vectorDepositorInsertFIS (fis E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only