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pIB184-Km

Plasmid

#90195

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

Type

Empty backbone

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDonorCm(Ptr)

Plasmid

#175577

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, Cm cassette

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-MCM4/6

Plasmid

#116951

Purpose

Expresses human MCM4 and human MCM6

Depositor

James Chong

Article

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM6 (MCM6 Human)
Human MCM4 (MCM4 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-T-GECO1

Plasmid

#191616

Purpose

Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

Depositor

Robert Campbell

Article

Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

Insert

T-GECO1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Virgina Zakian, Jean-Baptiste Boulé

Article

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Use

Tags

histag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSBK.079

Plasmid

#187218

Purpose

Expresses Eco1 RT and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 Reverse Transcriptase
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23115 and T7/lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2M

Plasmid

#15798

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2M (UBE2M Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
PAP-delta10-cHIS

Plasmid

#21716

Depositor

Andrew Bohm

Article

Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

Insert

polyA polymerase (PAP1 Budding Yeast)

Use

Tags

HIS

Expression

Bacterial

Mutation

30 amino acids off the c terminus

Promoter

Available since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only
pKM586

Plasmid

#19978

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease (TEVgp1 tobacco etch virus)

Use

Tags

Expression

Bacterial

Mutation

S219D mutant

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pUCP30T-E2Crimson

Plasmid

#78478

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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MINA

Plasmid

#39054

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MINA (RIOX2 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-Gm-ecfp

Plasmid

#65030

Purpose

GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCPP5108

Plasmid

#128726

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAA1-2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5107

Plasmid

#128725

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAA1-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0283 (pTnsABC)

Plasmid

#130633

Purpose

Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAG308-His-redFAST

Plasmid

#160350

Purpose

Expresses His-tagged redFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-redFAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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