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Plasmid#198599PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-143-144
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPhiC31o
Plasmid#13794PurposeExpression of mouse codon-optimized PhiC31 recombinaseDepositorInsertPhiC31o
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLRT
Plasmid#78234PurposeExpresses Murine Leukemia Reverse Transcriptase (MLRT)DepositorInsertMurine leukemia reverse transcriptase
UseTagsExpressionBacterialMutationPromoterJ23102Available sinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBL7010
Plasmid#136942PurposeConstitutive sfGFP expression (high), bacterial promoterDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKB01_mCherry
Plasmid#69104PurposeExpresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-10
Plasmid#172743PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-10
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE311B
Plasmid#120418PurposeExpresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanRDepositorArticleInsertsMutL E32K
Lambda Beta
XylS
UseTagsExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterPmAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GSK3 beta pGEX
Plasmid#15898DepositorInsertGSK3 beta (GSK3B Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP A1 RRM2ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV515
Plasmid#177703PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp9
Plasmid#167264PurposeBacterial expression of Nsp9DepositorInsertNsp9 (residues 1-113) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJKR-H-tetR
Plasmid#62561PurposeTetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM656
Plasmid#68887PurposeRhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
IVT-MCP-MMLV-RT
Plasmid#207457Purposein vitro transcription of mRNADepositorInsertMCP-RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pP2_tail_fiber
Plasmid#196332PurposePlasmid expressing P2 tail fiber H and chaperone protein GDepositorInsertP2 tail fiber H and chaperone protein G
UseTagsExpressionBacterialMutationPromoterP2 late promoterAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAmCyan1-C1-ADCY6
Plasmid#113905PurposeN-terminal Cyan Fluorescent tag in Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 (ADCY6 Human)
UseTagsCyanExpressionBacterial and MammalianMutationPromoterpCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS ColI alpha2
Plasmid#19207DepositorInsertAlpha 2 type I collagen (COL1A2 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only