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Plasmid#10861DepositorInsertubiquitin (UBI4 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTCF7 HLA-B*07:02
Plasmid#180446PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*07:02
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.1
Plasmid#42631DepositorInsertHuman H3.1 (H3C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEB1-SCFP3A
Plasmid#103970PurposePlasmid encoding SCFP3ADepositorInsertSCFP3A
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYFJ16-LplA(W37V) (for E. Coli expression)
Plasmid#34838DepositorInsertLplA W37V (lplA E. Coli)
UseTagsHis TagExpressionBacterialMutationMutated Trp37 to ValPromoterT5 promoterAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-F1
Plasmid#194592PurposeBacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSRK2721
Plasmid#78572PurposeGateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusionsDepositorInsertGateway cloning cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-BiFC
Plasmid#87856PurposepETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.DepositorInsertsEncodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus
UseTagsBiFC C-terminal fragmentExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL3287
Plasmid#139750PurposeBase Cas12a CRISPRi plasmidDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HisMBP-SED1
Plasmid#178191PurposeSED1 biosensor for expression in Escherichia coliDepositorInsertSED1 biosensor
UseTags6xHis-MBPExpressionBacterialMutationPromotertacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
UseTagsExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD
Plasmid#190089PurposeExpresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBadcrtEBI_13
Plasmid#63811Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET17_K560_GFP_His
Plasmid#15219DepositorInsertHuman neuronal kinesin heavy chain (KIF5B Human)
UseTagsGFP and HisExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPSU15
Plasmid#173828PurposeExpresses 15 kD Penn State ladder proteinDepositorInsertHSTPABPAC
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-mCherry
Plasmid#136615PurposeDox-inducible lentivral vector expressing mCherry red fluorescent proteinDepositorInsertmCherry
UseLentiviralTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Sox10
Plasmid#46536PurposeA lentiviral vector for the expression of mouse Sox10 under doxycyline control.DepositorInsertSox10 (Sox10 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only