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Strain: C321.ΔA

Bacterial Strain

#68306

Purpose

Genomically recoded strain of E. coli (no genomic TAG)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Bacterial Resistance

Bleocin (zeocin)

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
SJ358

Bacterial Strain

#67755

Purpose

a derivative of E. coli strain NCM3722 which has been cured of the F plasmid

Depositor

Suckjoon Jun

Article

Brown et al Genome Announc. 2015 Aug 6;3(4). pii: e0

Bacterial Resistance

None

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
BM28 ΔlysU

Bacterial Strain

#201048

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Kanamycin

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only
MG1655-tMMR

Bacterial Strain

#62392

Purpose

E. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expression

Depositor

Csaba Pál

Article

Nyerges et al Nucleic Acids Res. 2014 Apr;42(8):e62. d

Bacterial Resistance

Tetracycline

Species

Escherichia coli

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only
EcNR2.dnaG.Q576A

Bacterial Strain

#41700

Depositor

George Church

Article

Lajoie et al Nucleic Acids Res. 2012 Aug 16.

Bacterial Resistance

Ampicillin

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
BM28

Bacterial Strain

#201047

Purpose

MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.

Depositor

Jeannette Winter

Article

McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi

Bacterial Resistance

Ampicillin and kanamycin

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only
Nuc5-.dnaG.Q576A.tolC-

Bacterial Strain

#41699

Depositor

George Church

Article

Lajoie et al Nucleic Acids Res. 2012 Aug 16.

Bacterial Resistance

Ampicillin

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
C321.ΔA.M9adapted

Bacterial Strain

#98568

Purpose

Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP

Bacterial Strain

#182776

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.Ub-UAG-sfGFP

Bacterial Strain

#98564

Purpose

Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter

Depositor

George Church

Article

Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne- lon-

Bacterial Strain

#182778

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne-

Bacterial Strain

#182777

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.ΔClpS.Ub-UAG-sfGFP

Bacterial Strain

#98565

Purpose

Recoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivated

Depositor

George Church

Article

Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
EcM2.1.dtolC

Bacterial Strain

#64053

Purpose

Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

Depositor

George Church

Article

Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

Bacterial Resistance

Ampicillin

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
MCJ.559

Bacterial Strain

#69493

Purpose

Strain with recoded TAG and genomic deletions for improved incorporation

Depositor

Michael Jewett

Article

Hong et al Chembiochem. 2015 Mar 23;16(5):844-53. d

Bacterial Resistance

Ampicillin

Species

Eschericia coli

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
EcM2.1

Bacterial Strain

#64052

Purpose

Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

Depositor

George Church

Article

Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

Bacterial Resistance

Ampicillin

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
C321

Bacterial Strain

#48999

Purpose

Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 83 results

Results per page:

Sort results by:

Strain: C321.ΔA

C321.∆A.opt

SJ358

BM28 ΔlysU

MG1655-tMMR

EcNR2.dnaG.Q576A

BM28

Nuc5-.dnaG.Q576A.tolC-

C321.ΔA

C321.ΔA.M9adapted

C321.∆A T7RNAP

C321.Ub-UAG-sfGFP

C321.∆A T7RNAP ompT- rne- lon-

C321.∆A T7RNAP ompT- rne-

C321.ΔClpS.Ub-UAG-sfGFP

EcM2.1.dtolC

MCJ.559

EcM2.1

C321.ΔA.exp

C321

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