C321.ΔA.M9adapted
(Bacterial strain #98568)

• Purpose: Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin
• Depositing Lab: George Church
• Publication: Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3090-3095. doi: 10.1073/pnas.1715530115. Epub 2018 Feb 13.

Backbone

• Vector backbone: C321.deltaA genome after adaptive evolution in glucose minimal media (+ biotin)
• Backbone size w/o insert (bp): 4600000
• Modifications to backbone: The full sequence of this evolved strain is included on NCBI (accession number CP025268)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): C321.deltaA
• Growth instructions: The C321.deltaA strain contains an integrated lambda red cassette under expression by heat shock. Growth at 34 C or lower is recommended to prevent leaky expression of lambda red.
• Copy number: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry