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Plasmid#85598PurposeTol2 transposon with EGFP driven by minimal-CAGs promoter.DepositorInsertTol2 transposon with EGFP
UseTagsExpressionMammalianMutationPromotermini-CAGAvailable sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKTol2P-PTK
Plasmid#85599PurposeTol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.DepositorInsertTol2 transposon with a puromycin-thymidine kinase fusion
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKC-Tol2
Plasmid#85600PurposeSource of Tol2 transposase driven by a mini-CAGS promoter.DepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromotermini-CAGAvailable sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKUb-Tol2
Plasmid#85601PurposeSource of Tol2 transposase driven by a human ubiquitin promoter.DepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromoterhuman UbiquitinAvailable sinceFeb. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tol2
Plasmid#31823DepositorInsertTol2 Transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Tol2
Plasmid#31831DepositorInsertTol2 Transposase
UseCloning vectorTagsExpressionMutationPromoterLacAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2
Plasmid#31829DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAK_Tol2_TRE_Hygro
Plasmid#130260PurposeTol2 doxycycline inducible expression vectorDepositorTypeEmpty backboneUseTol2 transposon, doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAK_Tol2_TRE_Puro
Plasmid#130259PurposeTol2 doxycycline inducible expression vectorDepositorTypeEmpty backboneUseTol2 transposon, doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE PromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK_Tol2_TRE_Blast
Plasmid#130261PurposeTol2 doxycycline inducible expression vectorDepositorTypeEmpty backboneUseTol2 transposon, doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE PromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK_Tol2_TRE_Zeo
Plasmid#130263PurposeTol2 doxycycline inducible expression vectorDepositorTypeEmpty backboneUseTol2 transposon, doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE PromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK_Tol2_TRE_Neo
Plasmid#130262PurposeTol2 doxycycline inducible expression vectorDepositorTypeEmpty backboneUseTol2 transposon, doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE PromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-DNM1L -GFP
Plasmid#221845PurposeTol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterCAGC and U6.3 chickAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-MFN1-GFP
Plasmid#221846PurposeTol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promoteDepositorInsertsEGFP
MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterCAGC and U6.3 chickAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-non-targeting-control -GFP
Plasmid#221844PurposeTol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
non-targeting control sgRNA- GCACTGCTACGATCTACACC
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterGACG and U6.3 chickAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2_ubi:EGFP
Plasmid#27323DepositorInsertubiquitin:EGFP reporter
UseTol2 transposonTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-ubi-ASAP1
Plasmid#121987Purposeubiqutously express ASAP1 voltage biosensorDepositorInsertASAP1
UseTol2 transposon destination vectorTagsExpressionMutationPromoterzebrafish ubiquitin promoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-9xGRE
Plasmid#74736PurposeGRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elementsDepositorInsertEGFP
UseZebrafish expressionTagsExpressionMutationPromoternine GC-responsive elements (9xGRE) from the rat …Available sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-empty-GFP
Plasmid#221843Purposeempty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertEGFP
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterCAGCAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2-CreLite
Plasmid#131783PurposeTol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.DepositorInsertPhyBCreC, PIF6CreN, and mTagBFP2
UseCre/Lox and Synthetic Biology; Zebrafish tol2 tra…TagsmTagBFP2ExpressionMammalianMutationPromoterUbiAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only