pKUb-Tol2
(Plasmid
#85601)
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PurposeSource of Tol2 transposase driven by a human ubiquitin promoter.
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85601 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepK
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Backbone manufacturerK. Clark
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTol2 transposase
- Promoter human Ubiquitin
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer hUBCpro-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKUb-Tol2 was a gift from Karl Clark & Scott Fahrenkrug (Addgene plasmid # 85601 ; http://n2t.net/addgene:85601 ; RRID:Addgene_85601) -
For your References section:
Enzymatic engineering of the porcine genome with transposons and recombinases. Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC. BMC Biotechnol. 2007 Jul 17;7:42. 10.1186/1472-6750-7-42 PubMed 17640337