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Plasmid#61427Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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61427-sgHtt_10
Plasmid#157861PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_6
Plasmid#157860PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_zeo insert c-MYC
Plasmid#164636PurposesgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64DepositorInserttarget sequence for c-Myc activation (MYC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-start
Plasmid#127168PurposeEntry plasmid for two-step guide-barcode cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterN/AAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGH224_sgRNA_2xMS2_Puro
Plasmid#85413PurposehU6 driven sgRNA vector with 2xMS2 vectors with puro selectable markerDepositorInsertpuromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-EF1a-Puro
Plasmid#127170PurposePCR template for EF1a-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-CMV-Puro
Plasmid#127169PurposePCR template for CMV-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_TMv2
Plasmid#131761Purposeframeshift reporter for barcode retrievalDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_TMv2_Zeo
Plasmid#131762Purposeframeshift reporter for barcode retrieval (with Zeo selection)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorInsertGFP-RelA (RELA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO6A1_STOP
Plasmid#161427PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO6A1 (SLCO6A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMiR-RECK-3'UTR
Plasmid#53614PurposeLuciferase reporter assay for microRNA binding on the RECK 3'UTRDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
2xHA MED10 pBacPAK8
Plasmid#17321DepositorInsertMED10 (Med10 Mouse)
UseTags2xHAExpressionInsectMutationPromoterAvailable sinceMarch 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED10
Plasmid#15417DepositorInsertMED10 (Med10 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMiR-RECK-3'UTR-miR-21-mut
Plasmid#53693PurposeLuciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding siteDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseTagsExpressionMutationnucleotide sequence 1083-1086 is mutated to TTCGPromoterAvailable sinceJuly 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMiR-RECK-3'UTR-miR-7-mut
Plasmid#53692PurposeLuciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding siteDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseTagsExpressionMutationnucleotide position 171-174 are mutated to GAAGPromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human SLC SAM Library
Pooled Library#132561PurposeExpresses gRNAs for activation of human Solute Carrier family genesDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only