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Plasmid#48753PurposePiggyBac backbone, CAG promoter, Hygromycin selectionDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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pRoTnsABCDQ
Plasmid#178772PurposeExpression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalisDepositorInsertRotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRoTarget
Plasmid#178774Purposepartial tRNA-Leugene from Rippkaea orientalisDepositorInsertpartial tRNA-Leugene from Rippkaea orientalis
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2
Plasmid#220245PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-sumo-TEV-hAgo2 D597N
Plasmid#136236PurposeExpressing catalytically inactive human Argonaute-2 in insect cellsDepositorInserthuman Argonaute-2 (AGO2 Human)
UseTagsSterp SumoExpressionInsectMutationD597NPromoterpolyhedrinAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a2SE
Plasmid#49169PurposepHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-2 (GABRA2 Human)
UseTagsHA, bovine alpha 1 signal sequence, and pHGFP (pH…ExpressionMammalianMutationT343APromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-Fc(IgG1)
Plasmid#145165PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-sfGFP
Plasmid#145173PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-8his
Plasmid#149270PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pv6_MECP2_MBD-R106W
Plasmid#179408PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)DepositorInsertMECP2_MBD(R106W) (Mecp2 Mouse)
UseTagsAviTag and EGFPExpressionMammalianMutationMBD R106WPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2
Plasmid#145149PurposeMammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-Fc(IgA1)
Plasmid#145157PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgA1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pv6_MECP2_MBD-R133C
Plasmid#179409PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)DepositorInsertMECP2_MBD(R133C) (Mecp2 Mouse)
UseTagsAviTag and EGFPExpressionMammalianMutationMBD R133CPromoterCAGGSAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_MECP2_MBD-T158M
Plasmid#179410PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)DepositorInsertMECP2_MBD(T158M) (Mecp2 Mouse)
UseTagsAviTag and EGFPExpressionMammalianMutationMBD T158MPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_3KA + S580A POLI
Plasmid#131241PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580ADepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains point mutations that convert Pol iota se…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2(732)
Plasmid#154099PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MIIa
Plasmid#58684PurposeExpresses LGP2 with a mutation to motif IIa in mammalian cellsDepositorInsertLGP2-MIIa (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationmutation to Motif IIA, K138E and Y142FPromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-sACE2v2(732)-IgG1
Plasmid#154105PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2(732)-8h
Plasmid#154102PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only