pCMV6-AN-DDK_3KA + S580A POLI
(Plasmid #131241)

• Purpose: Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A
• Depositing Lab: Roger Woodgate
• Publication: Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. doi: 10.1016/j.jmb.2020.166733. Epub 2020 Dec 3.

Backbone

• Vector backbone: pCMV6-AN-DDK
• Backbone manufacturer: Origene
• Backbone size w/o insert (bp): 5876
• Total vector size (bp): 8066
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DNA polymerase iota
• Alt name: POLI
• Alt name: DNA polymerase iota isoform a (short)
• Species: H. sapiens (human)
• Insert Size (bp): 2190
• Mutation: Contains point mutations that convert Pol iota serine 580 to alanine (S580A), lysine residues 440, 444 and 446 to alanine (3KA), as well as introduce silent XhoI and KasI restriction sites. The pol iota CDS has been codon optimised for expression in human cells.
• GenBank ID: NM_001351632.2
• Entrez Gene: POLI (a.k.a. RAD30B, RAD3OB, eta2)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pCMV6-AN-DDK_3KA + S580A POLI.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry