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RHPMP139 (pLifeAct mEOS3.1)

Plasmid

#88839

Purpose

Localization by Super-Resolution microscopy of lifeAct fused to mOrange2

Depositor

Ricardo Henriques

Insert

LifeAct

Use

Tags

mEOS3.1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DrFLINC-AKAR-Kras

Plasmid

#182334

Purpose

Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

DrFLINC-AKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Dronpa contains H193T mutation.

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.1b K+ channel [N16B/8R]

Plasmid

#114511

Purpose

Mammalian Expression Plasmid of anti-Kv3.1b K+ channel (Rat). Derived from hybridoma N16B/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv3.1b K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnc1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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RHPMP190 (pLifeAct tagRFP-T)

Plasmid

#88842

Purpose

Localization by Fluorescence microscopy of lifeAct fused to tagRFP-T

Depositor

Ricardo Henriques

Insert

LifeAct

Use

Tags

tagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES-hSphK2-EGFP

Plasmid

#84371

Purpose

Expression of exogenous human SphK2 in mammalian cells

Depositor

Hugh Rosen, Anthony Don

Article

Don et al J Biol Chem. 2007 May 25;282(21):15833-4

Insert

SPHK2 (SPHK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only
pYES2.1-cerMET8

Plasmid

#107439

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET8 (MET8 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0026-mCherryBb

Plasmid

#118239

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0026 promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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p184_LTJ_sgRNACD90.2

Plasmid

#82670

Purpose

sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD90.2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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ER-CanlonicSF

Plasmid

#178343

Purpose

To explore endoplasmic reticulum lactate dynamics

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

ER-CanlonicSF

Use

Tags

ER Retention Signal, ER Signal Exon 1, and ER Sig…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDR FLIP43 GPS1

Plasmid

#101084

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Use

Tags

6HIS and cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-mRFP

Plasmid

#117781

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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GABPA_pLENTI-CAG-IRES-GFP

Plasmid

#176999

Purpose

Mammalian lentiviral expression vector encoding GABPA

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GABPA (GABPA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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mAmetrine-mStrawberry-FRET

Plasmid

#58170

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Type

Empty backbone

Use

Tags

mAmetrine and mStrawberry

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
pHR-OptoCAR-mScarlet

Plasmid

#170463

Purpose

Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

OptoCAR

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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DdMCU

Plasmid

#67642

Purpose

DdMCU-Flag in pACT2 vector human codon optimised

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

DdMCU

Use

Tags

Flag tag with linker

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dronpa-Kras

Plasmid

#182329

Purpose

Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Dronpa-Kras

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-Dronpa

Plasmid

#182332

Purpose

AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

AKAP79-Dronpa (AKAP5 Human)

Use

Tags

Full-length AKAP79

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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