-
Plasmid#221225PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporterDepositorInsertAID-PcrA M6-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE1062
Plasmid#172356PurposeDetection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.DepositorInsertsUseSynthetic Biology; Integrative into streptomyces …TagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-BLM-UGI-T2A-mTagBFP2
Plasmid#221222PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporterDepositorInsertAID-BLM-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tadDE-PcrA M6-UGI
Plasmid#221236PurposeExpress HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.DepositorInserttadDE-PcrA M6-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115268PurposeExpresses EiCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertEiCsm6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_XS3B-miniXon_Luciferase
Plasmid#217302PurposePlasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by LDepositorInsertXS3B-miniXon
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP943
Plasmid#104157PurposeBacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fab specific nanobody TP886
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
modified pcAT7-Glo1
Plasmid#160996PurposeSplicing reporter backboneDepositorInserthemoglobin subunit beta (HBB Human)
UseTagsExpressionMammalianMutationRepeated intron 1 with 40 nucelotide deletionPromoterCAGAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS8-HaloTagx2
Plasmid#177113PurposeExpresses Vps8-HaloTagx2 in yeast cellsDepositorInsertVPS8 (VPS8 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo VPS51-13Myc
Plasmid#175094Purpose13Myc tagged VPS51 is expressed in mammalian cellsDepositorInsertVPS51 (VPS51 Human)
UseTags13MycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLF063: Lenti-fCAG-jRGECO1a
Plasmid#124775PurposeFor general lentiviral or direct expression of cytosolic jRGECO1aDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianMutationPromoterCAGAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only