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Plasmid#160909PurposeRUBY under the control of Ubiquitin promoter/marker for transformationDepositorInsertRUBY and UBIQUITIN promoter
UseTagsExpressionPlantMutationPromoterUBIQUITINAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
UseTagsExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)
Viral Prep#75470-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)
Viral Prep#137128-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.TurboRFP.WPRE.RBG (AAV1)
Viral Prep#105552-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.hSyn.TurboRFP.WPRE.RBG (#105552). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.TurboRFP.WPRE.RBG plasmid DNA. hSynapsin-driven TurboRFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsTurboRFPAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bx
Plasmid#51552PurposeExpresses Bxb1 integrase in mammalian cells.DepositorInsertBxb1 integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUGW
Plasmid#14883Purpose3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresionDepositorInsertflap-Ub promoter-GFP-WRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1
Plasmid#193356PurposeVEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCsDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.3b (AAV5)
Viral Prep#125560-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag Rac2
Plasmid#12192DepositorInsertRac2 (RAC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKOR1
Plasmid#133446PurposeEscherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selectionDepositorTypeEmpty backboneUseE.coli/s.aureus shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-MAPPER
Plasmid#117721PurposeMAPPER Selectively Labels ER-PM JunctionsDepositorInsertMAPPER
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E283 Hs.ROCK1
Plasmid#70567PurposeGateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)DepositorInsertROCK1 (ROCK1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-L2-TALEN
Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorInsertH11-L2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
ER-mNeonGreen
Plasmid#137804PurposeVisualization of the Endoplasmic ReticulumDepositorArticleInsertKDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV8)
Viral Prep#28306-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b-PelB-6H-Ag43-160N-sfGFP
Plasmid#172886PurposeDisplay sfGFP protein on E. coli cell surfaceDepositorInsertsUseTagsHHHHHH and TEV recognition site (ENLYFQG)ExpressionBacterialMutationDeleted 1-160 (N terminal)PromoterT7 PromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only