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Plasmid#167961Purposeexpress E. coli E69 (O9a:K30) Wzc with Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y717E, Y718E
UseTagshexa-histidine tagExpressionBacterialMutationY717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN215
Plasmid#167962Purposeexpress E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
UseTagshexa-histidine tagExpressionBacterialMutationY715E, Y717E, Y718EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN261
Plasmid#167966Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
UseTagshexa-histidine tagExpressionBacterialMutationY713E, Y715E, Y717EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN081
Plasmid#173443Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y705EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y705E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y705EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN082
Plasmid#173444Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y706E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN083
Plasmid#173445Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y705E, Y706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN084
Plasmid#173446Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y708EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y708E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y708EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN085
Plasmid#173447Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y713EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN008
Plasmid#167949Purposeexpress E. coli E69 (O9a:K30) Wzc with K540M mutationDepositorInsertbacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
UseTagshexa-histidine tagExpressionBacterialMutationK540MPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN093
Plasmid#167951Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
UseTagshexa-histidine tagExpressionBacterialMutationK540M, delete motif 3PromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN074
Plasmid#167955Purposeexpress E. coli E69 (O9a:K30) Wzc with Y705E, Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y705E, Y706E
UseTagshexa-histidine tagExpressionBacterialMutationY705E, Y706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DYRK4_IDG-K
Plasmid#158184PurposeGateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DYRK4 (DYRK4 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CAMK1G_IDG-K
Plasmid#158168PurposeGateway destination clone of CAMK1G (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMK1G (CAMK1G Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140170PurposeExpresses a regulatable secretory cargo for Arabidopsis thalianaDepositorInsertpSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140169PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thalianaDepositorInsertpSpoA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140168PurposeExpresses a regulatable secretory cargo for Tetrahymena thermophilaDepositorInsertssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termiā¦ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Orange)
Plasmid#140157PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Orange)
Plasmid#140158PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Crimson)
Plasmid#140156PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only