Addgene

We narrowed to 8,125 results for: sgRNA

Showing: 1841 - 1860 of 8125 results

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pCas9-J23109

Plasmid

#113150

Purpose

Plasmid constitutively expressing Cas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T3_gRNA

Plasmid

#199335

Purpose

pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type III sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-Aa-artsg13 (BsaI)

Plasmid

#188279

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Aa-artsg13

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 (SpeI)

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HF1

Plasmid

#104173

Purpose

Cas9-HF1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-HF1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP2367

Plasmid

#160076

Purpose

Z. mobilis CRISPRi, promoter C, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMM100

Plasmid

#127225

Purpose

AtU6::sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6, miniCMV

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLY108

Plasmid

#130938

Purpose

Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plux2

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFH102

Plasmid

#128207

Purpose

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH85

Plasmid

#128198

Purpose

classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH86

Plasmid

#128199

Purpose

improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLY134

Plasmid

#130962

Purpose

Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB5

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Anderson promoter J23100

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFH88

Plasmid

#128201

Purpose

sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0058

Plasmid

#117539

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

sgRNA (SaCas9)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLY103

Plasmid

#130937

Purpose

Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plux2

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFH99

Plasmid

#128204

Purpose

classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH100

Plasmid

#128205

Purpose

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH101

Plasmid

#128206

Purpose

sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge