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  4. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid
    #164266
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
    Depositor
    Nikolaus Rajewsky
    Article
    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    U6 promoter from W05B2.8
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164266-plasmid-map-sequence-id-325780

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  5. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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  6. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    David Sabatini, Eric Lander
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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  13. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, PGK, and U6
    Available since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  15. T5-sgRNA

    Plasmid
    #113655
    Purpose
    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
    Depositor
    Jeffrey Barrick
    Article
    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531
    Insert
    promoter T5 and sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113655-plasmid-map-sequence-id-218814

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  17. pJ23119-sgRNA

    Plasmid
    #113654
    Purpose
    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
    Depositor
    Jeffrey Barrick
    Article
    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531
    Insert
    promoter J23119 and sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113654-plasmid-map-sequence-id-217170

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Showing: 1 - 20 of 7956 results