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Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Puro-Cas9 donor
Plasmid#58409PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locusDepositorInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H3
Plasmid#79514PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDSDepositorInsertLXR Alpha (NR1H3 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBK1289-AAV-dCjCas9
Plasmid#223138PurposeAAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorInsertsUseTags(EAAAK)8 linker and His6 + SUMOExpressionBacterialMutation2-116PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus
Plasmid#25734PurposeLentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
evoBxb1_IVT
Plasmid#222341PurposePlasmid for evolved Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved Bxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
deltaNp63alpha-FLAG
Plasmid#26979DepositorInsertΔNP63alpha (TP63 Human)
UseTagsFLAGExpressionMammalianMutationdominant negative isoform of p63; aa 1–587PromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
EP-Pol vector
Plasmid#131686PurposeA lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymeraseDepositorInsertAdenovirus DNA polymerase
UseLentiviralTagsHAExpressionMutationF421Y, D827APromoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo
Plasmid#25735Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1_IVT
Plasmid#222340PurposePlasmid for wild type Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized wtBxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterT7 (inactivated)Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Hygro
Plasmid#25737Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mKate2-B_UTR
Plasmid#68480PurposeLentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mKate2-B_UTR
UseLentiviralTagsExpressionMammalianMutationshRNA sensor cassette "B" added to 3…PromoterCMV and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-SynTetOff-GFP
Plasmid#190230PurposeGateway entry vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin Helper
Plasmid#127694PurposePlasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Glycoproteins
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits