Addgene

We narrowed to 5,672 results for: ttl

Showing: 1821 - 1840 of 5672 results

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pRN10

Plasmid

#84454

Purpose

shuttle plasmid for sarA P1-mKate expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mKate2

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRL25

Plasmid

#70253

Purpose

Shuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains

Depositor

Peter Wolk

Article

Wolk et al J Bacteriol. 1988 Mar;170(3):1239-44.

Type

Empty backbone

Use

Bacterial shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMSC130

Plasmid

#216364

Purpose

xhNup98-Nb3i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup98-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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Thy1.1-muSOX

Plasmid

#131702

Purpose

encodes Thy1.1 followed by a P2A site and muSOX for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-muSOX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDUP3

Plasmid

#92174

Purpose

Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

Depositor

Judith Berman

Article

Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

Insert

NAT1

Use

Genbank: u03440.1

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET19b+H3

Plasmid

#157807

Purpose

Expression of cysteine-free human histone H3 (c111A)

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H3

Use

Tags

Expression

Bacterial

Mutation

C111A

Promoter

T7

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Neil Fairweather, Robert Fagan

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTH1

Plasmid

#84451

Purpose

shuttle plasmid for sarA P1-dsRED expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

DsRed.T3(DNT)

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ140

Plasmid

#99895

Purpose

Empty vector with attL5-attL2 recombination sites for Gateway Cloning

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_p65_hsf1

Plasmid

#79374

Purpose

Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-p65-hsf1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS3

Plasmid

#109287

Purpose

Expresses proteins in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

New promoter/cloning site

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRL1383a

Plasmid

#70692

Purpose

RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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ECFP-TRAF2

Plasmid

#111201

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

TRAF2 (TRAF2 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1021

Plasmid

#108978

Purpose

shuttle vector, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Expression

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRPF144

Plasmid

#106372

Purpose

C. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusA

Depositor

Neil Fairweather, Robert Fagan

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Pcwp2-gusA (gusA )

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

codon optimised for C. difficile

Promoter

Pcwp2

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1020

Plasmid

#108977

Purpose

shuttle vector, C-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-muSOX

Plasmid

#131701

Purpose

expresses muSOX with GFP N-terminally fused

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

GFP-muSOX (GAMMAHV.ORF37 MHV68)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Mutation

Promoter

polH (polyhedrin)

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-TRAF2

Plasmid

#111202

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

TRAF2 (TRAF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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