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Plasmid#212079PurposeLR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cellsDepositorInsertZmynd8+exon(48nt) (Zmynd8 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorInsertproLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3526
Plasmid#145002PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTHAP12 (THAP12 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Wnk1
Plasmid#174874PurposeCRISPR vector for generating Wnk1 STREAMING-tag KI cellDepositorInsertsgRNA for mouse Wnk1 (Wnk1 Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα
Plasmid#183454PurposesgRNA targeting rat PKA-RIIα subunitsDepositorInsertsgRNA targeting rat PKA-RIIα (Prkar2a Rat)
UseLentiviralTagsExpressionMutationPromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-OLIG2-Adel-IDR
Plasmid#145249PurposeExpresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDRDepositorInsertOLIG2-Adel-IDR (OLIG2 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsGRK6[L66A E514A]-sYFP2
Plasmid#137781PurposeVisualization of GRK6DepositorInsertHuman GRK6[L66A E514A] (GRK6 Human)
UseTagsExpressionMammalianMutationL66A E514APromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11A)-mPAGFP
Plasmid#114134PurposeMammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN-mPAGFP
Plasmid#114114PurposeMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-PARK6e5-I368N
Plasmid#86154PurposeDonor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomatoDepositorInsertPINK1 exon 5 homology arms (PINK1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSi-check2-hRac2 3'UTR
Plasmid#97160PurposeFor dual luciferase report assayDepositorInsertRAC 3'UTR (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS
Plasmid#87776PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK-HygroDepositorInsertMKK4 (MAP2K4 Human)
UseTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MKNK2 gRNA (BRDN0001147215)
Plasmid#77762Purpose3rd generation lentiviral gRNA plasmid targeting human MKNK2DepositorInsertMKNK2 (MKNK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MKNK2 gRNA (BRDN0001145258)
Plasmid#77763Purpose3rd generation lentiviral gRNA plasmid targeting human MKNK2DepositorInsertMKNK2 (MKNK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MKNK2 gRNA (BRDN0001148143)
Plasmid#77764Purpose3rd generation lentiviral gRNA plasmid targeting human MKNK2DepositorInsertMKNK2 (MKNK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MKNK2 gRNA (BRDN0001148155)
Plasmid#77765Purpose3rd generation lentiviral gRNA plasmid targeting human MKNK2DepositorInsertMKNK2 (MKNK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
WNK3 gRNA (BRDN0001146119)
Plasmid#76925Purpose3rd generation lentiviral gRNA plasmid targeting human WNK3DepositorInsertWNK3 (WNK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
WNK3 gRNA (BRDN0001148134)
Plasmid#76926Purpose3rd generation lentiviral gRNA plasmid targeting human WNK3DepositorInsertWNK3 (WNK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
WNK3 gRNA (BRDN0001145371)
Plasmid#76927Purpose3rd generation lentiviral gRNA plasmid targeting human WNK3DepositorInsertWNK3 (WNK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only