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GB1-ΔN-CHMP4B(S184C)

Plasmid

#180026

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)

Use

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

S184C

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSKB2-TRex

Plasmid

#31349

Depositor

Clara Kielkopf

Article

McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

Insert

Thermus aquaticus Redox Sensing Repressor

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
V2rf2-IRES-BL Targeting Vector

Plasmid

#15860

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

V2rf2 (Vmn2r81 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
V2rf2 Southern Blot 5'-external probe

Plasmid

#15862

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

V2rf2 (Vmn2r81 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pT-36

Plasmid

#16252

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

CDC3 (CDC3 Budding Yeast)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pISH-bovine-tau

Plasmid

#15864

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

bovine tau (MAPT )

Use

Tags

Expression

Mutation

Promoter

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 CDK8 HA (P#634)

Plasmid

#14649

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk8 (CDK8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pAY02

Plasmid

#13043

Depositor

Peggy Hsieh

Article

Yamamoto et al Nucleic Acids Res. 2000 Sep 15. 28(18):3

Insert

MutS

Use

Tags

His6

Expression

Bacterial

Mutation

T193TT to G193CT Phe to Ala mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
M10.2 Southern Blot 5'-external probe

Plasmid

#15861

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

M10.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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M10.2::GFP-ACNF Targeting Vector

Plasmid

#15859

Depositor

Peter Mombaerts

Article

Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

Insert

M10.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 6, 2008

Availability

Academic Institutions and Nonprofits only
pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3b

Plasmid

#65613

Purpose

express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3b (csf3b Zebrafish)

Use

Tags

(His)6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only
pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-GFP

Plasmid

#127888

Purpose

Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

Puro-GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-mCherry

Plasmid

#127887

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

Puro-mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-H3FAID

Plasmid

#127884

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HF

Plasmid

#127882

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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dFP

Plasmid

#120542

Purpose

Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein (dFP)

Use

Tags

6xHis - TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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