Addgene

We narrowed to 7,600 results for: Dos

Showing: 161 - 180 of 7600 results

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pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA

Plasmid

#37086

Purpose

Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-YFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37082

Purpose

Cre-dependent expression of hChR2(H134R) for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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HA Display H3_A/Hong Kong/1/1968 Part 4-DO

Plasmid

#206518

Purpose

CcdB Dropout vector for part 4 of Hemagglutinin surface expression in mammalian cells

Depositor

IIya Finkelstein

Type

Empty backbone

Use

Tags

3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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HA Display H3_A/Hong Kong/1/1968 Part 1-DO

Plasmid

#206515

Purpose

CcdB Dropout vector for part 1 of Hemagglutinin surface expression in mammalian cells

Depositor

IIya Finkelstein

Type

Empty backbone

Use

Tags

3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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ylqF-pET21a

Plasmid

#30133

Depositor

SeWon Suh

Article

Kim do et al Proteins. 2008 Sep . 72(4):1363-70.

Insert

ylqF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pET28b-TM1570

Plasmid

#30162

Depositor

SeWon Suh

Article

Kim do et al Proteins. 2009 Jan . 74(1):245-9.

Insert

TM1570 (TM1570 Thermotoga maritima)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pET28b-LipB

Plasmid

#30165

Depositor

SeWon Suh

Article

Kim do et al Proteins. 2008 Mar . 70(4):1620-5.

Insert

lipoate protein ligase B

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Dest-pA-pgk-bsd

Plasmid

#74918

Purpose

Gateway destination piggyBac vector

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
pET28b-AF0623

Plasmid

#30164

Depositor

SeWon Suh

Article

Kim do et al Acta Crystallogr Sect F Struct Biol Crys

Insert

AF0623 (lig Archaeoglobus fulgidus)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
LX-miR

Pooled Library

#112200

Purpose

gRNA library targets human primary miRNAs

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only
Mouse Liver CRISPR Knockout Library

Pooled Library

#192824

Purpose

CRISPR screening library containing ~72,000 sgRNAs

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Mbd3b-pA-pgk-bsd

Plasmid

#74920

Purpose

Mammalian expression of Mbd3b

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mnd3b (Mbd3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Mbd3c-pA-pgk-bsd

Plasmid

#74921

Purpose

Mammalian expression of Mbd3c

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mbd3c (Mbd3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Klf4-pA-pgk-hph

Plasmid

#74906

Purpose

Mammalian expression of mKlf4

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Klf4 (Klf4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

Plasmid

#74919

Purpose

Mammalian expression of mKlf2 and mNanog-mCherry

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Inserts

Klf2 (Klf2 Mouse)
Nanog (Nanog Mouse)

Use

Tags

T2A and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
pTN182

Plasmid

#34888

Depositor

Mitsuyasu Hasebe

Article

Sakakibara et al Evol Dev. 2008 Sep-Oct;10(5):555-66.

Type

Empty backbone

Use

Moss targeting

Tags

Expression

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pGSH0

Plasmid

#45500

Depositor

Nelson Lau

Article

Zeng et al Methods. 2013 May 23. pii: S1046-2023(13

Type

Empty backbone

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pQC

Plasmid

#221134

Purpose

Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator

Depositor

Daniel Daley

Article

Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102

Inserts

[PibpA-gfpASV]
[PcpxPmcherryASV] OPT

Use

Tags

Expression

Bacterial

Mutation

Promoter

cpxP and ibpA

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pALOD4

Plasmid

#111026

Purpose

Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A

Promoter

T7

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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