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Plasmid#49375DepositorInsertLmrA repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-SacB-Cas9-T2A-mCherry_(BbsI)
Plasmid#117070PurposeEncodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selectionDepositorInsertsCas9
SacB
UseCRISPRTags3x FLAG, NLS, and T2A-mCherryExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF-HlyIIR
Plasmid#49361DepositorInsertHlyIIR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Pfrm_BsMdh_BmHps_BmPhi
Plasmid#129109PurposeExpresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoterDepositorInsertBsMDH BmHPS BmPHI
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCG-B-SpyTag-C
Plasmid#87373PurposeAn ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assemblyDepositorInsertSpyTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT440
Plasmid#8609DepositorInsertbarnase, barstar
UseTagsas pMT416ExpressionBacterialMutationV36 codon of barnase of pMT416 replaced by polyli…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BM3R1
Plasmid#51158DepositorInsertBM3R1
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOSV00170
Plasmid#222934PurposeConstitutive GFP expression for Bacillus subtilisDepositorInsertGFP(Sp)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNGsacB
Plasmid#203676PurposepQFT with mNeonGreen and sacB under control of PQJDepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_BAS1509
Plasmid#82354PurposeT7 in vitro transcription template for Bas mRNADepositorInsertBAS1509 hypothetical protein
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-HlyIIR
Plasmid#51174DepositorInsertHlyIIR
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Cyp106
Plasmid#51166DepositorInsertCyp106
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BM1P1
Plasmid#51157DepositorInsertBM1P1
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ4004
Plasmid#204401PurposeA 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsmCinR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PCin0102DAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHT-ads
Plasmid#47382PurposeExpresses ads under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
UseTags6xHisExpressionBacterialMutationPromoterPgracAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pALOD4
Plasmid#111026PurposeExpresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472ADepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472APromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPfetFn-Target_v2
Plasmid#199564PurposegRNA expression for FnCas12a in E. coli and clostridiaDepositorInsertSacB
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPthl_fUAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLN-AIDA-Tyr1
Plasmid#192831PurposeIPTG inducible AIDA-Tyrosinase expressionDepositorInsertAIDA-Tyr1
UseTagsExpressionBacterialMutationPromoterpL-lacAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mARG1 cassette 1
Plasmid#134343PurposeMammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.DepositorInsertmARG 1 cassete 1
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
mARG1 cassette 2
Plasmid#134344PurposeMammalian acoustic reporter gene 1 cassette 2 on the piggyBac trasposon plasmid.DepositorInsertmARG 1 cassete 2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only