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Plasmid#63106PurposeExpression of HSPB5 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb5 (CRYAB Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR162-Tango
Plasmid#66335PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR162 (GPR162 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
HTR4-DuET
Plasmid#213324PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR4 (HTR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE HA Chk2 KD, dominant negative chk2
Plasmid#41902DepositorInsertChk2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationD347APromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB8
Plasmid#63109PurposeExpression of HSPB8 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb8 (HSPB8 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#1
Plasmid#70095PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#2
Plasmid#70096PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoteru6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDUET-1-alpha-synuclein-mCerulean3-His6
Plasmid#110061PurposeFor bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidinesDepositorInsertalpha-synuclein (SNCA Human)
UseTags6 x His and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-OSF-PP-human-CHMP3
Plasmid#154180Purposeexpresses human CHMP3 in mammalian cellsDepositorInsertCHMP3 (CHMP3 Human)
UseTagsFLAG and Strep-Tag IIExpressionMammalianMutationPromoterCAGAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
MC2R-Tango
Plasmid#66428PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMC2R (MC2R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV5-B
Plasmid#74978PurposeshETV5 shRNADepositorInsertETV5 (ETV5 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.5 shETV5-A
Plasmid#74977PurposeshETV5 shRNADepositorInsertETV5 (ETV5 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 FL
Plasmid#58893PurposeExpresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.DepositorInsertTEM4 (ARHGEF17 Human)
UseTagsEGFPExpressionMammalianMutationL722FPromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-full-length-CHMP3-HA
Plasmid#154176Purposeexpresses mouse CHMP3 in mammalian cellsDepositorInsertCHMP3 (Chmp3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorInsertSNCA-GFP (SNCA Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorInsertHSPA5 promoter (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
Plasmid#52890Purpose"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.DepositorInsertsUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only