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Plasmid#219779PurposesTb(N) half of CaST for HEK cell expressionDepositorInsertCaM-V5-sTb(N)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hACE2-cMYC-Flag
Plasmid#158957PurposeUsed for expression of human ACE2 with AAV packagingDepositorInsertHuman ACE2 (ACE2 Human)
UseTagsFlag and MYCExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-PdCO-EGFP-WPRE
Plasmid#198513PurposeExpresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-EGFP-NLS
Plasmid#165441PurposeExpresses nuclear EGFP from CMV promoterDepositorInsertEGFP-NLS
UseAAVTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-EGFP-WPRE-pA
Plasmid#37085PurposeExpresses EGFP in cells lacking Cre (Cre-Off)DepositorInsertEGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-tdTomato
Plasmid#62726PurposeAAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-MCP-huADARE488QT490A
Plasmid#159922PurposeFusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5DepositorInsertHuman ADAR2 E488QT490A (ADARB1 Human)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IGK-dAPEX2-KDEL
Plasmid#117182PurposePlasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAVTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1596 - pAAV SYN1 HA-hM3D(Gq)
Plasmid#121539PurposeAn adeno-associated viral vector expressing HA-tagged stimulatory DREADD (hM3D(Gq)) from a synapsin promoterDepositorUseAAVTagsHAExpressionMammalianMutationPromoterSYN1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8f-WPRE
Plasmid#179254PurposeAAV-mediated expression of GCaMP8f under the CAG promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8f
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-CIAO-Flp
Plasmid#149294PurposeCre-dependent expression of Flp recombinase.DepositorInsertFlp recombinase (flp Budding Yeast)
UseAAVTagsExpressionMutationDerived from the mouse codon-optomized FlpO. The…PromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-EYFP-WPRE
Plasmid#104111PurposeAAV-mediated expression of EYFP under the TRE promoter.DepositorInsertEYFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8m
Plasmid#167574PurposePlease see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.DepositorInsertRibo-GCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.iAChSnFR-Venus-NULL
Plasmid#137960PurposeExpresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoterDepositorArticleInsertiAChSnFR (non-binding yellow variant)
UseAAV and Cre/LoxTagsExpressionMammalianMutationY140A binding site mutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
rAAV-U6-MeCP2shRNA-CamKIIa-mCherry
Plasmid#169704PurposeMeCP2 KnockdownDepositorInsertshRNA against MeCp2 (Mecp2 Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1-gs4_EF1a-Cas9-2A-GFP
Plasmid#118414PurposeCRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locusDepositorInsertgRNA AAVS1 (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
011LP-pAAV-TNT4-EmGFP-pA
Plasmid#120280PurposeAAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoterDepositorInsertEmGFP
UseAAVTags3XFlag and HAExpressionMutationPromoterTNT Cardiac Troponin T promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSyn1-EGFP
Plasmid#153187PurposeMouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DCX(S306D)-2A-mCherry
Plasmid#118292PurposeExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acidDepositorInsertDoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationSerine 306 changed to Aspartic AcidPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only